LOCUS       pAN581       4712 bp    DNA   circular             2-AUG-2007
DEFINITION  d35S->YFP.nos3'.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|287061842|
COMMENT     VNTDBDATE|453660416|
COMMENT     VNTNAME|pAN581|
COMMENT     VNTAUTHORNAME|NebenfŸhr Lab|
COMMENT     VNTAUTHORTEL|(865) 974-9201|
COMMENT     VNTAUTHORWWW|http://botany1.bio.utk.edu|
COMMENT     VNTAUTHORAD1|Univeristy of Tennessee|
COMMENT     VNTAUTHORAD2|Department of Botany|
COMMENT     VNTAUTHORAD3|1406 Circle Dr|
COMMENT     VNTAUTHORAD4|Knoxville, TN 37996-1100|
COMMENT     VNTOAUTHORNAME|Andreas NebenfŸhr|
COMMENT     VNTOAUTHORTEL|(865) 974-9201|
COMMENT     VNTOAUTHORFAX|(865) 974-2258|
COMMENT     VNTOAUTHOREML|nebenfuehr@utk.edu|
COMMENT     VNTOAUTHORWWW|http://botany1.bio.utk.edu/cellbiol|
COMMENT     VNTOAUTHORAD1|University of Tennessee|
COMMENT     VNTOAUTHORAD2|Department of Botany|
COMMENT     VNTOAUTHORAD3|437 Hesler Biology|
COMMENT     VNTOAUTHORAD4|Knoxville, TN 37996-1100|
FEATURES             Location/Qualifiers
     promoter        10..736
                     /vntifkey="29"
                     /label=d35S
                     /note="double 35S promoter"
     terminator      1554..1803
                     /vntifkey="43"
                     /label=nos\3'
                     /note="terminator from nopaline synthase gene"
     primer_bind     672..695
                     /vntifkey="28"
                     /label=35S\primer
     CDS             812..1534
                     /vntifkey="4"
                     /label=EYFP
                     /note="EYFP coding sequence"
     primer_bind     complement(865..887)
                     /vntifkey="28"
                     /label=SEQ-GFP-NR
                     /note="annealing site of GFP sequencing primer"
     primer_bind     1403..1422
                     /vntifkey="28"
                     /label=SEQ-YFP-F1
                     /note="primer is specific for YFP"
     primer_bind     complement(1007..1024)
                     /vntifkey="28"
                     /label=SEQ-YFP-R1
                     /note="primer is specific for YFP"
     primer          805..824
                     /vntifkey="27"
                     /label=YFP-F
     primer_bind     complement(1520..1536)
                     /vntifkey="28"
                     /label=YFP-R
BASE COUNT     1249 a      1214 c      1171 g      1078 t 
ORIGIN
        1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 
       61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 
      121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 
      181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 
      241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 
      301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 
      361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 
      421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 
      481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 
      541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 
      601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 
      661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 
      721 tcatttcatt tggagaggac agcccagatc aactagtctt aagtccggag ctagctctag 
      781 agacgtctcg aggaccggtc ccgggggatc catggtgagc aagggcgagg agctgttcac 
      841 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt 
      901 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 
      961 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctaca 
     1021 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 
     1081 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 
     1141 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 
     1201 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa 
     1261 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca 
     1321 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 
     1381 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa 
     1441 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 
     1501 cactctcggc atggacgagc tgtacagatc ttaaagcggc cgcccggctg cagttcaaac 
     1561 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata 
     1621 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt 
     1681 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac 
     1741 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat 
     1801 cgggaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc cggtacccag 
     1861 cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt 
     1921 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 
     1981 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 
     2041 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 
     2101 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 
     2161 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 
     2221 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 
     2281 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 
     2341 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 
     2401 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 
     2461 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 
     2521 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 
     2581 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 
     2641 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 
     2701 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 
     2761 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 
     2821 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 
     2881 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 
     2941 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 
     3001 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 
     3061 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 
     3121 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 
     3181 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 
     3241 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 
     3301 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 
     3361 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 
     3421 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 
     3481 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 
     3541 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 
     3601 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 
     3661 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 
     3721 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 
     3781 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 
     3841 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 
     3901 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 
     3961 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 
     4021 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt 
     4081 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 
     4141 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 
     4201 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 
     4261 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 
     4321 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 
     4381 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 
     4441 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 
     4501 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg 
     4561 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta 
     4621 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg 
     4681 taatacgact cactataggg cgaattggag ct 
//
