LOCUS       vac-yb      10380 bp    DNA   circular            18-OCT-2007
DEFINITION  pAN173.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|446562519|
COMMENT     VNTDBDATE|460549317|
COMMENT     VNTNAME|vac-yb|
COMMENT     VNTAUTHORNAME|NebenfŸhr Lab|
COMMENT     VNTAUTHORTEL|(865) 974-9201|
COMMENT     VNTAUTHORWWW|http://botany1.bio.utk.edu|
COMMENT     VNTAUTHORAD1|Univeristy of Tennessee|
COMMENT     VNTAUTHORAD2|Department of Botany|
COMMENT     VNTAUTHORAD3|1406 Circle Dr|
COMMENT     VNTAUTHORAD4|Knoxville, TN 37996-1100|
FEATURES             Location/Qualifiers
     repeat_region   9054..9080
                     /vntifkey="34"
                     /label=LB
                     /note="left border repeat from C58 T-DNA"
     polyA_signal    complement(9164..9415)
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="from mannopine synthase"
     gene            complement(9426..9977)
                     /gene="BAR"
                     /vntifkey="60"
     CDS             complement(9426..9977)
                     /gene="BAR"
                     /codon_start=1
                     /product="phosphinothricin acetyl transferase"
                     /protein_id="AAP78910.1"
                     /db_xref="GI:32265028"
                     /vntifkey="4"
                     /label=BAR-R
                     /note="basta resistance protein"
     promoter        complement(9983..10363)
                     /vntifkey="30"
                     /label=Promoter\(bar)
                     /note="mannopine synthase promoter"
     repeat_region   2776..2799
                     /vntifkey="34"
                     /label=RB
                     /note="right border repeat from C58 T-DNA"
     rep_origin      complement(3861..4861)
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="pVS1"
     rep_origin      complement(7264..7545)
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="pBR325"
     gene            complement(7835..8629)
                     /gene="aadA"
                     /vntifkey="60"
     CDS             complement(7835..8629)
                     /gene="aadA"
                     /function="kanamycin resistance"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAP78911.1"
                     /db_xref="GI:32265029"
                     /vntifkey="4"
                     /label=KAN-R
                     /note="aadA"
     primer_bind     673..696
                     /vntifkey="28"
                     /label=35S\primer
     promoter        10..736
                     /vntifkey="29"
                     /label=d35S
                     /note="double 35S promoter"
     CDS             788..1540
                     /vntifkey="4"
                     /label=gammaTIP
                     /note="Arabidopsis gamma TIP ORF (At2g36830)"
     terminator      2287..2536
                     /vntifkey="43"
                     /label=nos\3'
                     /note="terminator from nopaline synthase gene"
     CDS             1547..2266
                     /vntifkey="4"
                     /label=EYFP
                     /note="EYFP coding sequence"
     primer_bind     complement(1600..1622)
                     /vntifkey="28"
                     /label=SEQ-GFP-NR
                     /note="annealing site of GFP sequencing primer"
BASE COUNT     2483 a      2887 c      2793 g      2217 t 
ORIGIN
        1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 
       61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 
      121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 
      181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 
      241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 
      301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 
      361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 
      421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 
      481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 
      541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 
      601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 
      661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 
      721 tcatttcatt tggagaggac agcccagatc aactagtctt aagtccggag ctagctctag 
      781 actcgagatg ccgatcagaa acatcgccat tggccgtcca gatgaagcca cccgtcccga 
      841 tgccttaaag gcggcgttgg ctgagttcat ttcaactttg atctttgtcg tcgccggttc 
      901 aggctctggc atggctttca acaagctcac tgaaaacgga gccaccactc cttctggtct 
      961 cgtagctgct gcagtggctc atgcctttgg actcttcgtc gctgtctcag ttggtgccaa 
     1021 catctctggt ggacacgtta accctgccgt cactttcggt gctttcattg gtggtaacat 
     1081 cactctcctc cgtggtatcc tctactggat tgctcagctt ctcggctccg tcgtcgcttg 
     1141 cctcatcctt aaattcgcca ccggtggctt ggctgtgccg gcttttggtc tctctgctgg 
     1201 agtaggagtg ttgaacgctt tcgttttcga gatcgtgatg acattcgggc ttgtttacac 
     1261 cgtctacgct acagccattg accccaaaaa cgggagtctt ggaacaattg ctcccatcgc 
     1321 aatcggtttc attgttggag ccaacatctt agctggagga gctttctctg gagcctccat 
     1381 gaatcccgcc gtggctttcg gaccagcggt ggtgagctgg acatggacca accactgggt 
     1441 ctactgggcc ggacctctcg tcggcggtgg aatcgctgga ctcatctacg aagttttctt 
     1501 catcaacacc acacacgagc agctcccaac cacagactac ggatccatgg tgagcaaggg 
     1561 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 
     1621 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 
     1681 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt 
     1741 cggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt 
     1801 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 
     1861 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 
     1921 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 
     1981 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa 
     2041 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 
     2101 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca 
     2161 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 
     2221 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcccggc 
     2281 tgcagttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg 
     2341 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc 
     2401 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac 
     2461 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct 
     2521 atgttactag atcgggaatt cgatatcaag cttggcactg gccgtcgttt tacaacgtcg 
     2581 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc 
     2641 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct 
     2701 gaatggcgaa tgctagagca gcttgagctt ggatcagatt gtcgtttccc gccttcagtt 
     2761 taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta 
     2821 ttagaataac ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt 
     2881 gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc 
     2941 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac 
     3001 aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg 
     3061 tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac 
     3121 aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg 
     3181 accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc 
     3241 accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc 
     3301 gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac 
     3361 attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc 
     3421 gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc 
     3481 gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc 
     3541 gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg 
     3601 atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg 
     3661 accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc 
     3721 ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa 
     3781 cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta 
     3841 ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg 
     3901 tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct 
     3961 ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat 
     4021 ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac 
     4081 aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg 
     4141 caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct 
     4201 gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca 
     4261 accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg 
     4321 ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc 
     4381 gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc 
     4441 caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca 
     4501 agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga 
     4561 ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta 
     4621 cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc 
     4681 ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt 
     4741 aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc 
     4801 agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt 
     4861 cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc 
     4921 attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata 
     4981 aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg 
     5041 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg 
     5101 ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg 
     5161 acggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg 
     5221 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg 
     5281 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag 
     5341 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc 
     5401 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc 
     5461 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc 
     5521 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac 
     5581 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca 
     5641 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg 
     5701 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg 
     5761 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg 
     5821 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg 
     5881 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc 
     5941 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc 
     6001 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac 
     6061 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt 
     6121 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag 
     6181 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc 
     6241 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg 
     6301 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga 
     6361 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa 
     6421 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg 
     6481 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc 
     6541 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct 
     6601 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc 
     6661 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt 
     6721 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 
     6781 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc 
     6841 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc 
     6901 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 
     6961 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 
     7021 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 
     7081 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 
     7141 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 
     7201 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 
     7261 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 
     7321 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 
     7381 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 
     7441 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 
     7501 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 
     7561 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 
     7621 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 
     7681 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 
     7741 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 
     7801 cacgttaagg gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat 
     7861 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat 
     7921 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact 
     7981 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa 
     8041 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg 
     8101 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt 
     8161 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt 
     8221 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact 
     8281 ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa 
     8341 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca 
     8401 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca 
     8461 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt 
     8521 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt 
     8581 atttttcgat cagttttttc aattccggtg atattctcat tttagccatt tattatttcc 
     8641 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc 
     8701 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt 
     8761 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc 
     8821 acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg 
     8881 tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa 
     8941 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta 
     9001 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg 
     9061 atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt 
     9121 ttaatgtact gaattaacgc cgaattaatt cgagctcgga tctgataatt tatttgaaaa 
     9181 ttcataagaa aagcaaacgt tacatgaatt gatgaaacaa tacaaagaca gataaagcca 
     9241 cgcacattta ggatattggc cgagattact gaatattgag taagatcacg gaatttctga 
     9301 caggagcatg tcttcaattc agcccaaatg gcagttgaaa tactcaaacc gccccatatg 
     9361 caggagcgga tcattcattg tttgtttggt tgcctttgcc aacatgggag tccaagattc 
     9421 tgcagttaga tctcggtgac gggcaggacc ggacggggcg gtaccggcag gctgaagtcc 
     9481 agctgccaga aacccacgtc atgccagttc ccgtgcttga agccggccgc ccgcagcatg 
     9541 ccgcgggggg catatccgag cgcctcgtgc atgcgcacgc tcgggtcgtt gggcagcccg 
     9601 atgacagcga ccacgctctt gaagccctgt gcctccaggg acttcagcag gtgggtgtag 
     9661 agcgtggagc ccagtcccgt ccgctggtgg cggggggaga cgtacacggt cgactcggcc 
     9721 gtccagtcgt aggcgttgcg tgccttccag gggcccgcgt aggcgatgcc ggcgacctcg 
     9781 ccgtccacct cggcgacgag ccagggatag cgctcccgca gacggacgag gtcgtccgtc 
     9841 cactcctgcg gttcctgcgg ctcggtacgg aagttgaccg tgcttgtctc gatgtagtgg 
     9901 ttgacgatgg tgcagaccgc cggcatgtcc gcctcggtgg cacggcggat gtcggccggg 
     9961 cgtcgttctg ggctcatcga ttcgatttgg tgtatcgaga ttggttatga aattcagatg 
    10021 ctagtgtaat gtattggtaa tttgggaaga tataatagga agcaaggcta tttatccatt 
    10081 tctgaaaagg cgaaatggcg tcaccgcgag cgtcacgcgc attccgttct tgctgtaaag 
    10141 cgttgtttgg tacacttttg actagcgagg cttggcgtgt cagcgtatct attcaaaagt 
    10201 cgttaatggc tgcggatcaa gaaaaagttg gaatagaaac agaatacccg cgaaattcag 
    10261 gcccggttgc catgtcctac acgccgaaat aaacgaccaa attagtagaa aaataaaaac 
    10321 tagctcagat acttacgtca cgtcttgcgc actgatttga aaaatctcag aattcgagct 
//
