LOCUS vac-yb 10380 bp DNA circular 18-OCT-2007 DEFINITION pAN173. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|446562519| COMMENT VNTDBDATE|460549317| COMMENT VNTNAME|vac-yb| COMMENT VNTAUTHORNAME|NebenfŸhr Lab| COMMENT VNTAUTHORTEL|(865) 974-9201| COMMENT VNTAUTHORWWW|http://botany1.bio.utk.edu| COMMENT VNTAUTHORAD1|Univeristy of Tennessee| COMMENT VNTAUTHORAD2|Department of Botany| COMMENT VNTAUTHORAD3|1406 Circle Dr| COMMENT VNTAUTHORAD4|Knoxville, TN 37996-1100| FEATURES Location/Qualifiers repeat_region 9054..9080 /vntifkey="34" /label=LB /note="left border repeat from C58 T-DNA" polyA_signal complement(9164..9415) /vntifkey="25" /label=PolyA_Signal_1 /note="from mannopine synthase" gene complement(9426..9977) /gene="BAR" /vntifkey="60" CDS complement(9426..9977) /gene="BAR" /codon_start=1 /product="phosphinothricin acetyl transferase" /protein_id="AAP78910.1" /db_xref="GI:32265028" /vntifkey="4" /label=BAR-R /note="basta resistance protein" promoter complement(9983..10363) /vntifkey="30" /label=Promoter\(bar) /note="mannopine synthase promoter" repeat_region 2776..2799 /vntifkey="34" /label=RB /note="right border repeat from C58 T-DNA" rep_origin complement(3861..4861) /vntifkey="33" /label=Rep_Origin_1 /note="pVS1" rep_origin complement(7264..7545) /vntifkey="33" /label=Rep_Origin_2 /note="pBR325" gene complement(7835..8629) /gene="aadA" /vntifkey="60" CDS complement(7835..8629) /gene="aadA" /function="kanamycin resistance" /codon_start=1 /product="aminoglycoside phosphotransferase" /protein_id="AAP78911.1" /db_xref="GI:32265029" /vntifkey="4" /label=KAN-R /note="aadA" primer_bind 673..696 /vntifkey="28" /label=35S\primer promoter 10..736 /vntifkey="29" /label=d35S /note="double 35S promoter" CDS 788..1540 /vntifkey="4" /label=gammaTIP /note="Arabidopsis gamma TIP ORF (At2g36830)" terminator 2287..2536 /vntifkey="43" /label=nos\3' /note="terminator from nopaline synthase gene" CDS 1547..2266 /vntifkey="4" /label=EYFP /note="EYFP coding sequence" primer_bind complement(1600..1622) /vntifkey="28" /label=SEQ-GFP-NR /note="annealing site of GFP sequencing primer" BASE COUNT 2483 a 2887 c 2793 g 2217 t ORIGIN 1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 721 tcatttcatt tggagaggac agcccagatc aactagtctt aagtccggag ctagctctag 781 actcgagatg ccgatcagaa acatcgccat tggccgtcca gatgaagcca cccgtcccga 841 tgccttaaag gcggcgttgg ctgagttcat ttcaactttg atctttgtcg tcgccggttc 901 aggctctggc atggctttca acaagctcac tgaaaacgga gccaccactc cttctggtct 961 cgtagctgct gcagtggctc atgcctttgg actcttcgtc gctgtctcag ttggtgccaa 1021 catctctggt ggacacgtta accctgccgt cactttcggt gctttcattg gtggtaacat 1081 cactctcctc cgtggtatcc tctactggat tgctcagctt ctcggctccg tcgtcgcttg 1141 cctcatcctt aaattcgcca ccggtggctt ggctgtgccg gcttttggtc tctctgctgg 1201 agtaggagtg ttgaacgctt tcgttttcga gatcgtgatg acattcgggc ttgtttacac 1261 cgtctacgct acagccattg accccaaaaa cgggagtctt ggaacaattg ctcccatcgc 1321 aatcggtttc attgttggag ccaacatctt agctggagga gctttctctg gagcctccat 1381 gaatcccgcc gtggctttcg gaccagcggt ggtgagctgg acatggacca accactgggt 1441 ctactgggcc ggacctctcg tcggcggtgg aatcgctgga ctcatctacg aagttttctt 1501 catcaacacc acacacgagc agctcccaac cacagactac ggatccatgg tgagcaaggg 1561 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 1621 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 1681 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt 1741 cggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt 1801 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 1861 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 1921 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 1981 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa 2041 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 2101 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca 2161 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 2221 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcccggc 2281 tgcagttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg 2341 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc 2401 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac 2461 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct 2521 atgttactag atcgggaatt cgatatcaag cttggcactg gccgtcgttt tacaacgtcg 2581 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc 2641 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct 2701 gaatggcgaa tgctagagca gcttgagctt ggatcagatt gtcgtttccc gccttcagtt 2761 taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta 2821 ttagaataac ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt 2881 gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc 2941 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac 3001 aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg 3061 tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac 3121 aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg 3181 accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc 3241 accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc 3301 gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac 3361 attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc 3421 gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc 3481 gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc 3541 gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg 3601 atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg 3661 accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc 3721 ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa 3781 cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta 3841 ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg 3901 tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct 3961 ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat 4021 ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac 4081 aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg 4141 caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct 4201 gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca 4261 accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg 4321 ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc 4381 gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc 4441 caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca 4501 agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga 4561 ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta 4621 cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc 4681 ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt 4741 aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc 4801 agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt 4861 cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc 4921 attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata 4981 aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg 5041 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg 5101 ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg 5161 acggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg 5221 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg 5281 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag 5341 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc 5401 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc 5461 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc 5521 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac 5581 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca 5641 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg 5701 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg 5761 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg 5821 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg 5881 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc 5941 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc 6001 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac 6061 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt 6121 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag 6181 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc 6241 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg 6301 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga 6361 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa 6421 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg 6481 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc 6541 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct 6601 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc 6661 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt 6721 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 6781 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc 6841 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc 6901 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 6961 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 7021 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 7081 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 7141 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 7201 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 7261 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 7321 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 7381 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 7441 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 7501 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 7561 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 7621 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 7681 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 7741 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 7801 cacgttaagg gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat 7861 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat 7921 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact 7981 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa 8041 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg 8101 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt 8161 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt 8221 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact 8281 ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa 8341 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca 8401 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca 8461 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt 8521 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt 8581 atttttcgat cagttttttc aattccggtg atattctcat tttagccatt tattatttcc 8641 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc 8701 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt 8761 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc 8821 acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg 8881 tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa 8941 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta 9001 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg 9061 atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt 9121 ttaatgtact gaattaacgc cgaattaatt cgagctcgga tctgataatt tatttgaaaa 9181 ttcataagaa aagcaaacgt tacatgaatt gatgaaacaa tacaaagaca gataaagcca 9241 cgcacattta ggatattggc cgagattact gaatattgag taagatcacg gaatttctga 9301 caggagcatg tcttcaattc agcccaaatg gcagttgaaa tactcaaacc gccccatatg 9361 caggagcgga tcattcattg tttgtttggt tgcctttgcc aacatgggag tccaagattc 9421 tgcagttaga tctcggtgac gggcaggacc ggacggggcg gtaccggcag gctgaagtcc 9481 agctgccaga aacccacgtc atgccagttc ccgtgcttga agccggccgc ccgcagcatg 9541 ccgcgggggg catatccgag cgcctcgtgc atgcgcacgc tcgggtcgtt gggcagcccg 9601 atgacagcga ccacgctctt gaagccctgt gcctccaggg acttcagcag gtgggtgtag 9661 agcgtggagc ccagtcccgt ccgctggtgg cggggggaga cgtacacggt cgactcggcc 9721 gtccagtcgt aggcgttgcg tgccttccag gggcccgcgt aggcgatgcc ggcgacctcg 9781 ccgtccacct cggcgacgag ccagggatag cgctcccgca gacggacgag gtcgtccgtc 9841 cactcctgcg gttcctgcgg ctcggtacgg aagttgaccg tgcttgtctc gatgtagtgg 9901 ttgacgatgg tgcagaccgc cggcatgtcc gcctcggtgg cacggcggat gtcggccggg 9961 cgtcgttctg ggctcatcga ttcgatttgg tgtatcgaga ttggttatga aattcagatg 10021 ctagtgtaat gtattggtaa tttgggaaga tataatagga agcaaggcta tttatccatt 10081 tctgaaaagg cgaaatggcg tcaccgcgag cgtcacgcgc attccgttct tgctgtaaag 10141 cgttgtttgg tacacttttg actagcgagg cttggcgtgt cagcgtatct attcaaaagt 10201 cgttaatggc tgcggatcaa gaaaaagttg gaatagaaac agaatacccg cgaaattcag 10261 gcccggttgc catgtcctac acgccgaaat aaacgaccaa attagtagaa aaataaaaac 10321 tagctcagat acttacgtca cgtcttgcgc actgatttga aaaatctcag aattcgagct //