LOCUS       vac-rb      10371 bp    DNA   circular            18-OCT-2007
DEFINITION  pAN611.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|443790721|
COMMENT     VNTDBDATE|460549219|
COMMENT     VNTNAME|vac-rb|
COMMENT     VNTAUTHORNAME|NebenfŸhr Lab|
COMMENT     VNTAUTHORTEL|(865) 974-9201|
COMMENT     VNTAUTHORWWW|http://botany1.bio.utk.edu|
COMMENT     VNTAUTHORAD1|Univeristy of Tennessee|
COMMENT     VNTAUTHORAD2|Department of Botany|
COMMENT     VNTAUTHORAD3|1406 Circle Dr|
COMMENT     VNTAUTHORAD4|Knoxville, TN 37996-1100|
FEATURES             Location/Qualifiers
     repeat_region   9045..9071
                     /vntifkey="34"
                     /label=LB
                     /note="left border repeat from C58 T-DNA"
     polyA_signal    complement(9155..9406)
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="from mannopine synthase"
     gene            complement(9417..9968)
                     /gene="BAR"
                     /vntifkey="60"
     CDS             complement(9417..9968)
                     /gene="BAR"
                     /codon_start=1
                     /product="phosphinothricin acetyl transferase"
                     /protein_id="AAP78910.1"
                     /db_xref="GI:32265028"
                     /vntifkey="4"
                     /label=BAR-R
                     /note="basta resistance protein"
     promoter        complement(9974..10354)
                     /vntifkey="30"
                     /label=Promoter\(bar)
                     /note="mannopine synthase promoter"
     repeat_region   2767..2790
                     /vntifkey="34"
                     /label=RB
                     /note="right border repeat from C58 T-DNA"
     rep_origin      complement(3852..4852)
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="pVS1"
     rep_origin      complement(7255..7536)
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="pBR325"
     gene            complement(7826..8620)
                     /gene="aadA"
                     /vntifkey="60"
     CDS             complement(7826..8620)
                     /gene="aadA"
                     /function="kanamycin resistance"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAP78911.1"
                     /db_xref="GI:32265029"
                     /vntifkey="4"
                     /label=KAN-R
                     /note="aadA"
     primer_bind     2288..2307
                     /vntifkey="28"
                     /label=seq-NOS3-R
     primer_bind     673..696
                     /vntifkey="28"
                     /label=35S\primer
     promoter        10..736
                     /vntifkey="29"
                     /label=d35S
                     /note="double 35S promoter"
     terminator      2278..2527
                     /vntifkey="43"
                     /label=nos\3'
                     /note="terminator from nopaline synthase gene"
     CDS             788..1540
                     /vntifkey="4"
                     /label=gammaTIP
                     /note="Arabidopsis gamma TIP ORF (At2g36830)"
     CDS             1547..2257
                     /vntifkey="4"
                     /label=mCherry
BASE COUNT     2476 a      2872 c      2810 g      2213 t 
ORIGIN
        1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 
       61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 
      121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 
      181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 
      241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 
      301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 
      361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 
      421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 
      481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 
      541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 
      601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 
      661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 
      721 tcatttcatt tggagaggac agcccagatc aactagtctt aagtccggag ctagctctag 
      781 actcgagatg ccgatcagaa acatcgccat tggccgtcca gatgaagcca cccgtcccga 
      841 tgccttaaag gcggcgttgg ctgagttcat ttcaactttg atctttgtcg tcgccggttc 
      901 aggctctggc atggctttca acaagctcac tgaaaacgga gccaccactc cttctggtct 
      961 cgtagctgct gcagtggctc atgcctttgg actcttcgtc gctgtctcag ttggtgccaa 
     1021 catctctggt ggacacgtta accctgccgt cactttcggt gctttcattg gtggtaacat 
     1081 cactctcctc cgtggtatcc tctactggat tgctcagctt ctcggctccg tcgtcgcttg 
     1141 cctcatcctt aaattcgcca ccggtggctt ggctgtgccg gcttttggtc tctctgctgg 
     1201 agtaggagtg ttgaacgctt tcgttttcga gatcgtgatg acattcgggc ttgtttacac 
     1261 cgtctacgct acagccattg accccaaaaa cgggagtctt ggaacaattg ctcccatcgc 
     1321 aatcggtttc attgttggag ccaacatctt agctggagga gctttctctg gagcctccat 
     1381 gaatcccgcc gtggctttcg gaccagcggt ggtgagctgg acatggacca accactgggt 
     1441 ctactgggcc ggacctctcg tcggcggtgg aatcgctgga ctcatctacg aagttttctt 
     1501 catcaacacc acacacgagc agctcccaac cacagactac ggatccatgg tgagcaaggg 
     1561 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg 
     1621 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 
     1681 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat 
     1741 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc 
     1801 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 
     1861 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta 
     1921 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac 
     1981 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga 
     2041 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac 
     2101 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga 
     2161 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg 
     2221 ccactccacc ggcggcatgg acgagctgta caagtaaagc ggccgcccgg ctgcagttca 
     2281 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc 
     2341 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta 
     2401 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa 
     2461 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta 
     2521 gatcgggaat tcgatatcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga 
     2581 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 
     2641 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 
     2701 atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc 
     2761 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa 
     2821 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa 
     2881 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca 
     2941 gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa 
     3001 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt 
     3061 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc 
     3121 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa 
     3181 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc 
     3241 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg 
     3301 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag 
     3361 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc 
     3421 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc 
     3481 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt 
     3541 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag 
     3601 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac 
     3661 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc 
     3721 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag 
     3781 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga 
     3841 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg 
     3901 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca 
     3961 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa 
     4021 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca 
     4081 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac 
     4141 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga 
     4201 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac 
     4261 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga 
     4321 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc 
     4381 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga 
     4441 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt 
     4501 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc 
     4561 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac 
     4621 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt 
     4681 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa 
     4741 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct 
     4801 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg 
     4861 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag 
     4921 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag 
     4981 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc 
     5041 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt 
     5101 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gacggtcgca 
     5161 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga 
     5221 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt 
     5281 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc 
     5341 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct 
     5401 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga 
     5461 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg 
     5521 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg 
     5581 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc 
     5641 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa 
     5701 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc 
     5761 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga 
     5821 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc 
     5881 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc 
     5941 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc 
     6001 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca 
     6061 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc 
     6121 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct 
     6181 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag 
     6241 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt 
     6301 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc 
     6361 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt 
     6421 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat 
     6481 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc 
     6541 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg 
     6601 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc 
     6661 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc 
     6721 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga 
     6781 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag 
     6841 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac 
     6901 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 
     6961 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 
     7021 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 
     7081 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 
     7141 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 
     7201 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 
     7261 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 
     7321 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 
     7381 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 
     7441 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 
     7501 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 
     7561 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 
     7621 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 
     7681 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 
     7741 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 
     7801 ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta 
     7861 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt 
     7921 ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc 
     7981 tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc 
     8041 tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa 
     8101 aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca 
     8161 catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat 
     8221 tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca 
     8281 gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat 
     8341 cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg 
     8401 gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg 
     8461 tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca 
     8521 gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga 
     8581 tcagtttttt caattccggt gatattctca ttttagccat ttattatttc cttcctcttt 
     8641 tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact 
     8701 gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa 
     8761 gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc 
     8821 aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa 
     8881 cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc 
     8941 cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt 
     9001 gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt 
     9061 ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac 
     9121 tgaattaacg ccgaattaat tcgagctcgg atctgataat ttatttgaaa attcataaga 
     9181 aaagcaaacg ttacatgaat tgatgaaaca atacaaagac agataaagcc acgcacattt 
     9241 aggatattgg ccgagattac tgaatattga gtaagatcac ggaatttctg acaggagcat 
     9301 gtcttcaatt cagcccaaat ggcagttgaa atactcaaac cgccccatat gcaggagcgg 
     9361 atcattcatt gtttgtttgg ttgcctttgc caacatggga gtccaagatt ctgcagttag 
     9421 atctcggtga cgggcaggac cggacggggc ggtaccggca ggctgaagtc cagctgccag 
     9481 aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat gccgcggggg 
     9541 gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc gatgacagcg 
     9601 accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta gagcgtggag 
     9661 cccagtcccg tccgctggtg gcggggggag acgtacacgg tcgactcggc cgtccagtcg 
     9721 taggcgttgc gtgccttcca ggggcccgcg taggcgatgc cggcgacctc gccgtccacc 
     9781 tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt ccactcctgc 
     9841 ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg gttgacgatg 
     9901 gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg gcgtcgttct 
     9961 gggctcatcg attcgatttg gtgtatcgag attggttatg aaattcagat gctagtgtaa 
    10021 tgtattggta atttgggaag atataatagg aagcaaggct atttatccat ttctgaaaag 
    10081 gcgaaatggc gtcaccgcga gcgtcacgcg cattccgttc ttgctgtaaa gcgttgtttg 
    10141 gtacactttt gactagcgag gcttggcgtg tcagcgtatc tattcaaaag tcgttaatgg 
    10201 ctgcggatca agaaaaagtt ggaatagaaa cagaataccc gcgaaattca ggcccggttg 
    10261 ccatgtccta cacgccgaaa taaacgacca aattagtaga aaaataaaaa ctagctcaga 
    10321 tacttacgtc acgtcttgcg cactgatttg aaaaatctca gaattcgagc t 
//
