LOCUS       px-yb        9722 bp    DNA   circular            25-SEP-2007
DEFINITION  pAN594.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|446565842|
COMMENT     VNTDBDATE|458404366|
COMMENT     VNTNAME|px-yb|
COMMENT     VNTAUTHORNAME|NebenfŸhr Lab|
COMMENT     VNTAUTHORTEL|(865) 974-9201|
COMMENT     VNTAUTHORWWW|http://botany1.bio.utk.edu|
COMMENT     VNTAUTHORAD1|Univeristy of Tennessee|
COMMENT     VNTAUTHORAD2|Department of Botany|
COMMENT     VNTAUTHORAD3|1406 Circle Dr|
COMMENT     VNTAUTHORAD4|Knoxville, TN 37996-1100|
FEATURES             Location/Qualifiers
     repeat_region   8406..8432
                     /vntifkey="34"
                     /label=LB
                     /note="left border repeat from C58 T-DNA"
     polyA_signal    complement(8516..8767)
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="from mannopine synthase"
     gene            complement(8778..9329)
                     /gene="BAR"
                     /vntifkey="60"
     CDS             complement(8778..9329)
                     /gene="BAR"
                     /codon_start=1
                     /product="phosphinothricin acetyl transferase"
                     /protein_id="AAP78910.1"
                     /db_xref="GI:32265028"
                     /vntifkey="4"
                     /label=BAR-R
                     /note="basta resistance protein"
     promoter        complement(9335..9715)
                     /vntifkey="30"
                     /label=Promoter\(bar)
                     /note="mannopine synthase promoter"
     repeat_region   2128..2151
                     /vntifkey="34"
                     /label=RB
                     /note="right border repeat from C58 T-DNA"
     rep_origin      complement(3213..4213)
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="pVS1"
     rep_origin      complement(6616..6897)
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="pBR325"
     gene            complement(7187..7981)
                     /gene="aadA"
                     /vntifkey="60"
     CDS             complement(7187..7981)
                     /gene="aadA"
                     /function="kanamycin resistance"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAP78911.1"
                     /db_xref="GI:32265029"
                     /vntifkey="4"
                     /label=KAN-R
                     /note="aadA"
     CDS             complement(259..990)
                     /vntifkey="4"
                     /label=YFP-SKL
     promoter        complement(1134..1888)
                     /vntifkey="30"
                     /label=d35S
                     /note="cauliflower mosaic virus dual 35S promoter"
     5'UTR           complement(992..1133)
                     /vntifkey="52"
                     /label=5'UTR_1
                     /note="translational enhancer; 5'UTR from tobacco etch virus"
     terminator      complement(40..250)
                     /vntifkey="43"
                     /label=Terminator_1
                     /note="cauliflower mosaic virus 35S terminator"
BASE COUNT     2231 a      2608 c      2683 g      2200 t 
ORIGIN
        1 aattcgagct cggtacccgg ggatcccccg ggctgcaggt cactggattt tggttttagg 
       61 aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta agggtttctt 
      121 atatgctcaa cacatgagcg aaaccctata agaaccctaa ttcccttatc tgggaactac 
      181 tcacacatta ttctggagaa aaatagagag agatagattt gtagagagag actggtgatt 
      241 tttgcggact ctagatcttt acagcttcga tctcttgtac agctcgtcca tgccgagagt 
      301 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc 
      361 tttgctcagg gcggactggt agctcaggta gtggttgtcg ggcagcagca cggggccgtc 
      421 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt 
      481 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac 
      541 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa 
      601 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc 
      661 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc 
      721 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca 
      781 ctgcaggccg tagccgaagg tggtcacgag ggtgggccag ggcacgggca gcttgccggt 
      841 ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga 
      901 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc 
      961 ggtgaacagc tcctcgccct tgctcaccat ggctatcgtt cgtaaatggt gaaaattttc 
     1021 agaaaattgc ttttgcttta aaagaaatga tttaaattgc tgcaatagaa gtagaatgct 
     1081 tgattgcttg agattcgttt gttttgtata tgttgtgttg agaattctcg aggtcctctc 
     1141 caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc 
     1201 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg 
     1261 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc 
     1321 ggcagaggca tcttcaacga tggcctttcc tttatcgcaa tgatggcatt tgtaggagcc 
     1381 accttccttt tccactatct tcacaataaa gtgacagata gctgggcaat ggaatccgag 
     1441 gaggtttccg gatattaccc tttgttgaaa agtctcaatt gccctttggt cttctgagac 
     1501 tgtatctttg atatttttgg agtagacaag tgtgtcgtgc tccaccatgt tatcacatca 
     1561 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg 
     1621 tgggggtcca tctttgggac cactgtcggc agaggcatct tcaacgatgg cctttccttt 
     1681 atcgcaatga tggcatttgt aggagccacc ttccttttcc actatcttca caataaagtg 
     1741 acagatagct gggcaatgga atccgaggag gtttccggat attacccttt gttgaaaagt 
     1801 ctcaattgcc ctttggtctt ctgagactgt atctttgata tttttggagt agacaagtgt 
     1861 gtcgtgctcc accatgttga cctgcaggaa ttcgatatca agcttggcac tggccgtcgt 
     1921 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca 
     1981 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca 
     2041 gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc 
     2101 ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga 
     2161 aaagagcgtt tattagaata acggatattt aaaagggcgt gaaaaggttt atccgttcgt 
     2221 ccatttgtat gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa 
     2281 cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac 
     2341 gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt 
     2401 tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat 
     2461 tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac 
     2521 gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt 
     2581 tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac 
     2641 ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc 
     2701 gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca 
     2761 gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc 
     2821 attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc 
     2881 aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac 
     2941 gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc 
     3001 gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag 
     3061 gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc 
     3121 gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac 
     3181 cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc 
     3241 cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca 
     3301 agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa 
     3361 ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat 
     3421 gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa 
     3481 aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg 
     3541 ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt 
     3601 gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt 
     3661 gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt 
     3721 ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta 
     3781 cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga 
     3841 tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg 
     3901 tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca 
     3961 gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga 
     4021 gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg 
     4081 agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg 
     4141 agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag 
     4201 cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc 
     4261 caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg 
     4321 agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca 
     4381 agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc 
     4441 aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga 
     4501 ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg 
     4561 atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag 
     4621 aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc 
     4681 aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag 
     4741 attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg 
     4801 tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc 
     4861 ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt 
     4921 acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag 
     4981 ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct 
     5041 gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa 
     5101 acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg 
     5161 tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc 
     5221 cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga 
     5281 acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt 
     5341 ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga 
     5401 cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca 
     5461 agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg 
     5521 gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct 
     5581 aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc 
     5641 tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc 
     5701 cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata 
     5761 taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta 
     5821 aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag 
     5881 cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg 
     5941 ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag 
     6001 ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc 
     6061 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg 
     6121 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt 
     6181 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg 
     6241 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat 
     6301 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc 
     6361 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 
     6421 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 
     6481 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 
     6541 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 
     6601 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
     6661 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 
     6721 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
     6781 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
     6841 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 
     6901 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 
     6961 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 
     7021 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 
     7081 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 
     7141 ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtactaa aacaattcat 
     7201 ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa 
     7261 atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa 
     7321 tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc 
     7381 catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt 
     7441 cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt 
     7501 cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg 
     7561 ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga 
     7621 gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact 
     7681 cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc 
     7741 gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct 
     7801 tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata 
     7861 ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt 
     7921 ttacgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca 
     7981 tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa 
     8041 caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc 
     8101 tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa 
     8161 accgcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg 
     8221 cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt 
     8281 aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat 
     8341 gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg 
     8401 gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca 
     8461 ttgcggacgt ttttaatgta ctgaattaac gccgaattaa ttcgagctcg gatctgataa 
     8521 tttatttgaa aattcataag aaaagcaaac gttacatgaa ttgatgaaac aatacaaaga 
     8581 cagataaagc cacgcacatt taggatattg gccgagatta ctgaatattg agtaagatca 
     8641 cggaatttct gacaggagca tgtcttcaat tcagcccaaa tggcagttga aatactcaaa 
     8701 ccgccccata tgcaggagcg gatcattcat tgtttgtttg gttgcctttg ccaacatggg 
     8761 agtccaagat tctgcagtta gatctcggtg acgggcagga ccggacgggg cggtaccggc 
     8821 aggctgaagt ccagctgcca gaaacccacg tcatgccagt tcccgtgctt gaagccggcc 
     8881 gcccgcagca tgccgcgggg ggcatatccg agcgcctcgt gcatgcgcac gctcgggtcg 
     8941 ttgggcagcc cgatgacagc gaccacgctc ttgaagccct gtgcctccag ggacttcagc 
     9001 aggtgggtgt agagcgtgga gcccagtccc gtccgctggt ggcgggggga gacgtacacg 
     9061 gtcgactcgg ccgtccagtc gtaggcgttg cgtgccttcc aggggcccgc gtaggcgatg 
     9121 ccggcgacct cgccgtccac ctcggcgacg agccagggat agcgctcccg cagacggacg 
     9181 aggtcgtccg tccactcctg cggttcctgc ggctcggtac ggaagttgac cgtgcttgtc 
     9241 tcgatgtagt ggttgacgat ggtgcagacc gccggcatgt ccgcctcggt ggcacggcgg 
     9301 atgtcggccg ggcgtcgttc tgggctcatc gattcgattt ggtgtatcga gattggttat 
     9361 gaaattcaga tgctagtgta atgtattggt aatttgggaa gatataatag gaagcaaggc 
     9421 tatttatcca tttctgaaaa ggcgaaatgg cgtcaccgcg agcgtcacgc gcattccgtt 
     9481 cttgctgtaa agcgttgttt ggtacacttt tgactagcga ggcttggcgt gtcagcgtat 
     9541 ctattcaaaa gtcgttaatg gctgcggatc aagaaaaagt tggaatagaa acagaatacc 
     9601 cgcgaaattc aggcccggtt gccatgtcct acacgccgaa ataaacgacc aaattagtag 
     9661 aaaaataaaa actagctcag atacttacgt cacgtcttgc gcactgattt gaaaaatctc 
     9721 ag 
//
