LOCUS       px-gb        9713 bp    DNA   circular            18-OCT-2007
DEFINITION  pAN592.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|442853968|
COMMENT     VNTDBDATE|460548676|
COMMENT     VNTNAME|px-gb|
COMMENT     VNTAUTHORNAME|NebenfŸhr Lab|
COMMENT     VNTAUTHORTEL|(865) 974-9201|
COMMENT     VNTAUTHORWWW|http://botany1.bio.utk.edu|
COMMENT     VNTAUTHORAD1|Univeristy of Tennessee|
COMMENT     VNTAUTHORAD2|Department of Botany|
COMMENT     VNTAUTHORAD3|1406 Circle Dr|
COMMENT     VNTAUTHORAD4|Knoxville, TN 37996-1100|
FEATURES             Location/Qualifiers
     repeat_region   6518..6544
                     /vntifkey="34"
                     /label=LB
                     /note="left border repeat from C58 T-DNA"
     polyA_signal    complement(6628..6879)
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="from mannopine synthase"
     gene            complement(6890..7441)
                     /gene="BAR"
                     /vntifkey="60"
     CDS             complement(6890..7441)
                     /gene="BAR"
                     /codon_start=1
                     /product="phosphinothricin acetyl transferase"
                     /protein_id="AAP78910.1"
                     /db_xref="GI:32265028"
                     /vntifkey="4"
                     /label=BAR-R
                     /note="basta resistance protein"
     promoter        complement(7447..7827)
                     /vntifkey="30"
                     /label=Promoter\(bar)
                     /note="mannopine synthase promoter"
     repeat_region   240..263
                     /vntifkey="34"
                     /label=RB
                     /note="right border repeat from C58 T-DNA"
     rep_origin      complement(1325..2325)
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="pVS1"
     rep_origin      complement(4728..5009)
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="pBR325"
     gene            complement(5299..6093)
                     /gene="aadA"
                     /vntifkey="60"
     CDS             complement(5299..6093)
                     /gene="aadA"
                     /function="kanamycin resistance"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAP78911.1"
                     /db_xref="GI:32265029"
                     /vntifkey="4"
                     /label=KAN-R
                     /note="aadA"
     CDS             complement(8093..8815)
                     /vntifkey="4"
                     /label=GFP-SKL
                     /note="GFP+SKL"
     promoter        complement(8959..9713)
                     /vntifkey="30"
                     /label=Promoter_P_1
                     /note="cauliflower mosaic virus dual 35S promoter"
     5'UTR           complement(8817..8958)
                     /vntifkey="52"
                     /label=5'UTR_1
                     /note="translational enhancer; 5'UTR from tobacco etch virus"
     terminator      complement(7874..8084)
                     /vntifkey="43"
                     /label=Terminator_1
                     /note="cauliflower mosaic virus 35S terminator"
BASE COUNT     2293 a      2561 c      2614 g      2245 t 
ORIGIN
        1 aattcgatat caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct 
       61 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc 
      121 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgctag 
      181 agcagcttga gcttggatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt 
      241 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taacggatat 
      301 ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg 
      361 gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct 
      421 tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc 
      481 gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa 
      541 agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg 
      601 gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg 
      661 aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg 
      721 accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga 
      781 ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc 
      841 aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg 
      901 ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca 
      961 tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc 
     1021 gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc 
     1081 gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac 
     1141 ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg 
     1201 acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag 
     1261 catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc 
     1321 ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct 
     1381 gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc 
     1441 cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt 
     1501 gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa 
     1561 cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca 
     1621 acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat 
     1681 ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc 
     1741 ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta 
     1801 gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac 
     1861 ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg 
     1921 gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg 
     1981 tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac 
     2041 gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc 
     2101 gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg 
     2161 ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca 
     2221 aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt 
     2281 tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg 
     2341 atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat 
     2401 ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt 
     2461 ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa 
     2521 tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg 
     2581 ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgacggtc gcaaaccatc 
     2641 cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc 
     2701 gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc 
     2761 ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat 
     2821 taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt 
     2881 gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga 
     2941 ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc 
     3001 agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca 
     3061 tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt 
     3121 ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa 
     3181 agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac 
     3241 gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg 
     3301 ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga 
     3361 ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga 
     3421 ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc 
     3481 aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg 
     3541 agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga 
     3601 gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa 
     3661 cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat 
     3721 tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca cgtacattgg 
     3781 gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat 
     3841 tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc 
     3901 ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc 
     3961 tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct 
     4021 acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct 
     4081 acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg 
     4141 cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 
     4201 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 
     4261 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta 
     4321 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt 
     4381 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 
     4441 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     4501 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     4561 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     4621 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     4681 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     4741 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     4801 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     4861 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     4921 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     4981 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     5041 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 
     5101 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     5161 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
     5221 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 
     5281 ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc 
     5341 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat 
     5401 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct 
     5461 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc 
     5521 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata 
     5581 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc 
     5641 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata 
     5701 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat 
     5761 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc 
     5821 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg 
     5881 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc 
     5941 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata 
     6001 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt 
     6061 tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag 
     6121 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt 
     6181 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg 
     6241 tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc 
     6301 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca 
     6361 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa 
     6421 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg 
     6481 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa 
     6541 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta 
     6601 acgccgaatt aattcgagct cggatctgat aatttatttg aaaattcata agaaaagcaa 
     6661 acgttacatg aattgatgaa acaatacaaa gacagataaa gccacgcaca tttaggatat 
     6721 tggccgagat tactgaatat tgagtaagat cacggaattt ctgacaggag catgtcttca 
     6781 attcagccca aatggcagtt gaaatactca aaccgcccca tatgcaggag cggatcattc 
     6841 attgtttgtt tggttgcctt tgccaacatg ggagtccaag attctgcagt tagatctcgg 
     6901 tgacgggcag gaccggacgg ggcggtaccg gcaggctgaa gtccagctgc cagaaaccca 
     6961 cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc 
     7021 cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc 
     7081 tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg gagcccagtc 
     7141 ccgtccgctg gtggcggggg gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt 
     7201 tgcgtgcctt ccaggggccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga 
     7261 cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct 
     7321 gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg atggtgcaga 
     7381 ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggctca 
     7441 tcgattcgat ttggtgtatc gagattggtt atgaaattca gatgctagtg taatgtattg 
     7501 gtaatttggg aagatataat aggaagcaag gctatttatc catttctgaa aaggcgaaat 
     7561 ggcgtcaccg cgagcgtcac gcgcattccg ttcttgctgt aaagcgttgt ttggtacact 
     7621 tttgactagc gaggcttggc gtgtcagcgt atctattcaa aagtcgttaa tggctgcgga 
     7681 tcaagaaaaa gttggaatag aaacagaata cccgcgaaat tcaggcccgg ttgccatgtc 
     7741 ctacacgccg aaataaacga ccaaattagt agaaaaataa aaactagctc agatacttac 
     7801 gtcacgtctt gcgcactgat ttgaaaaatc tcagaattcg agctcggtac ccggggatcc 
     7861 cccgggctgc aggtcactgg attttggttt taggaattag aaattttatt gatagaagta 
     7921 ttttacaaat acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc 
     7981 tataagaacc ctaattccct tatctgggaa ctactcacac attattctgg agaaaaatag 
     8041 agagagatag atttgtagag agagactggt gatttttgcg gactctagat ctttacagct 
     8101 tcgatctgta tagttcatcc atgccatgtg taatcccagc agctgttaca aactcaagaa 
     8161 ggaccatgtg gtctctcttt tcgttgggat ctttcgaaag ggcagattgt gtggacaggt 
     8221 aatggttgtc tggtaaaagg acagggccat cgccaattgg agtattttgt tgataatgat 
     8281 cagcgagttg cacgccgccg tcttcgatgt tgtggcgggt cttgaagttg gctttgatgc 
     8341 cgttcttttg cttgtcggcc atgatgtata cgttgtggga gttgtagttg tattccaact 
     8401 tgtggccgag gatgtttccg tcctccttga aatcgattcc cttaagctcg atcctgttga 
     8461 cgagggtgtc tccctcaaac ttgacttcag cacgtgtctt gtagttcccg tcgtccttga 
     8521 agaagatggt cctctcctgc acgtatccct caggcatggc gctcttgaag aagtcgtgcc 
     8581 gcttcatatg atctgggtat cttgaaaagc attgaacacc ataagagaaa gtagtgacaa 
     8641 gtgttggcca gggaacaggt agttttccag tagtgcaaat aaatttaagg gtaagttttc 
     8701 cgtatgttgc atcaccttca ccctctccac tgacagaaaa tttgtgccca ttaacatcac 
     8761 catctaattc aacaagaatt gggacaactc cagtgaaaag ttctccttta cccatggcta 
     8821 tcgttcgtaa atggtgaaaa ttttcagaaa attgcttttg ctttaaaaga aatgatttaa 
     8881 attgctgcaa tagaagtaga atgcttgatt gcttgagatt cgtttgtttt gtatatgttg 
     8941 tgttgagaat tctcgaggtc ctctccaaat gaaatgaact tccttatata gaggaagggt 
     9001 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat 
     9061 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg 
     9121 ggggtccatc tttgggacca ctgtcggcag aggcatcttc aacgatggcc tttcctttat 
     9181 cgcaatgatg gcatttgtag gagccacctt ccttttccac tatcttcaca ataaagtgac 
     9241 agatagctgg gcaatggaat ccgaggaggt ttccggatat taccctttgt tgaaaagtct 
     9301 caattgccct ttggtcttct gagactgtat ctttgatatt tttggagtag acaagtgtgt 
     9361 cgtgctccac catgttatca catcaatcca cttgctttga agacgtggtt ggaacgtctt 
     9421 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg 
     9481 catcttcaac gatggccttt cctttatcgc aatgatggca tttgtaggag ccaccttcct 
     9541 tttccactat cttcacaata aagtgacaga tagctgggca atggaatccg aggaggtttc 
     9601 cggatattac cctttgttga aaagtctcaa ttgccctttg gtcttctgag actgtatctt 
     9661 tgatattttt ggagtagaca agtgtgtcgt gctccaccat gttgacctgc agg 
//
