LOCUS px-gb 9713 bp DNA circular 18-OCT-2007 DEFINITION pAN592. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|442853968| COMMENT VNTDBDATE|460548676| COMMENT VNTNAME|px-gb| COMMENT VNTAUTHORNAME|NebenfŸhr Lab| COMMENT VNTAUTHORTEL|(865) 974-9201| COMMENT VNTAUTHORWWW|http://botany1.bio.utk.edu| COMMENT VNTAUTHORAD1|Univeristy of Tennessee| COMMENT VNTAUTHORAD2|Department of Botany| COMMENT VNTAUTHORAD3|1406 Circle Dr| COMMENT VNTAUTHORAD4|Knoxville, TN 37996-1100| FEATURES Location/Qualifiers repeat_region 6518..6544 /vntifkey="34" /label=LB /note="left border repeat from C58 T-DNA" polyA_signal complement(6628..6879) /vntifkey="25" /label=PolyA_Signal_1 /note="from mannopine synthase" gene complement(6890..7441) /gene="BAR" /vntifkey="60" CDS complement(6890..7441) /gene="BAR" /codon_start=1 /product="phosphinothricin acetyl transferase" /protein_id="AAP78910.1" /db_xref="GI:32265028" /vntifkey="4" /label=BAR-R /note="basta resistance protein" promoter complement(7447..7827) /vntifkey="30" /label=Promoter\(bar) /note="mannopine synthase promoter" repeat_region 240..263 /vntifkey="34" /label=RB /note="right border repeat from C58 T-DNA" rep_origin complement(1325..2325) /vntifkey="33" /label=Rep_Origin_1 /note="pVS1" rep_origin complement(4728..5009) /vntifkey="33" /label=Rep_Origin_2 /note="pBR325" gene complement(5299..6093) /gene="aadA" /vntifkey="60" CDS complement(5299..6093) /gene="aadA" /function="kanamycin resistance" /codon_start=1 /product="aminoglycoside phosphotransferase" /protein_id="AAP78911.1" /db_xref="GI:32265029" /vntifkey="4" /label=KAN-R /note="aadA" CDS complement(8093..8815) /vntifkey="4" /label=GFP-SKL /note="GFP+SKL" promoter complement(8959..9713) /vntifkey="30" /label=Promoter_P_1 /note="cauliflower mosaic virus dual 35S promoter" 5'UTR complement(8817..8958) /vntifkey="52" /label=5'UTR_1 /note="translational enhancer; 5'UTR from tobacco etch virus" terminator complement(7874..8084) /vntifkey="43" /label=Terminator_1 /note="cauliflower mosaic virus 35S terminator" BASE COUNT 2293 a 2561 c 2614 g 2245 t ORIGIN 1 aattcgatat caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct 61 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc 121 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgctag 181 agcagcttga gcttggatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt 241 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taacggatat 301 ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg 361 gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct 421 tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc 481 gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa 541 agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg 601 gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg 661 aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg 721 accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga 781 ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc 841 aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg 901 ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca 961 tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc 1021 gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc 1081 gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac 1141 ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg 1201 acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag 1261 catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc 1321 ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct 1381 gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc 1441 cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt 1501 gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa 1561 cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca 1621 acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat 1681 ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc 1741 ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta 1801 gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac 1861 ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg 1921 gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg 1981 tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac 2041 gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc 2101 gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg 2161 ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca 2221 aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt 2281 tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg 2341 atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat 2401 ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt 2461 ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa 2521 tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg 2581 ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgacggtc gcaaaccatc 2641 cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc 2701 gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc 2761 ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat 2821 taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt 2881 gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga 2941 ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc 3001 agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca 3061 tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt 3121 ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa 3181 agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac 3241 gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg 3301 ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga 3361 ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga 3421 ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc 3481 aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg 3541 agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga 3601 gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa 3661 cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat 3721 tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca cgtacattgg 3781 gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat 3841 tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc 3901 ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc 3961 tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct 4021 acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct 4081 acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg 4141 cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 4201 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 4261 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta 4321 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt 4381 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 4441 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 4501 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 4561 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 4621 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 4681 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 4741 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 4801 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 4861 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 4921 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 4981 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 5041 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 5101 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 5161 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 5221 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 5281 ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc 5341 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat 5401 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct 5461 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc 5521 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata 5581 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc 5641 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata 5701 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat 5761 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc 5821 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg 5881 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc 5941 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata 6001 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt 6061 tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag 6121 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt 6181 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg 6241 tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc 6301 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca 6361 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa 6421 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg 6481 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa 6541 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta 6601 acgccgaatt aattcgagct cggatctgat aatttatttg aaaattcata agaaaagcaa 6661 acgttacatg aattgatgaa acaatacaaa gacagataaa gccacgcaca tttaggatat 6721 tggccgagat tactgaatat tgagtaagat cacggaattt ctgacaggag catgtcttca 6781 attcagccca aatggcagtt gaaatactca aaccgcccca tatgcaggag cggatcattc 6841 attgtttgtt tggttgcctt tgccaacatg ggagtccaag attctgcagt tagatctcgg 6901 tgacgggcag gaccggacgg ggcggtaccg gcaggctgaa gtccagctgc cagaaaccca 6961 cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc 7021 cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc 7081 tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg gagcccagtc 7141 ccgtccgctg gtggcggggg gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt 7201 tgcgtgcctt ccaggggccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga 7261 cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct 7321 gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg atggtgcaga 7381 ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggctca 7441 tcgattcgat ttggtgtatc gagattggtt atgaaattca gatgctagtg taatgtattg 7501 gtaatttggg aagatataat aggaagcaag gctatttatc catttctgaa aaggcgaaat 7561 ggcgtcaccg cgagcgtcac gcgcattccg ttcttgctgt aaagcgttgt ttggtacact 7621 tttgactagc gaggcttggc gtgtcagcgt atctattcaa aagtcgttaa tggctgcgga 7681 tcaagaaaaa gttggaatag aaacagaata cccgcgaaat tcaggcccgg ttgccatgtc 7741 ctacacgccg aaataaacga ccaaattagt agaaaaataa aaactagctc agatacttac 7801 gtcacgtctt gcgcactgat ttgaaaaatc tcagaattcg agctcggtac ccggggatcc 7861 cccgggctgc aggtcactgg attttggttt taggaattag aaattttatt gatagaagta 7921 ttttacaaat acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc 7981 tataagaacc ctaattccct tatctgggaa ctactcacac attattctgg agaaaaatag 8041 agagagatag atttgtagag agagactggt gatttttgcg gactctagat ctttacagct 8101 tcgatctgta tagttcatcc atgccatgtg taatcccagc agctgttaca aactcaagaa 8161 ggaccatgtg gtctctcttt tcgttgggat ctttcgaaag ggcagattgt gtggacaggt 8221 aatggttgtc tggtaaaagg acagggccat cgccaattgg agtattttgt tgataatgat 8281 cagcgagttg cacgccgccg tcttcgatgt tgtggcgggt cttgaagttg gctttgatgc 8341 cgttcttttg cttgtcggcc atgatgtata cgttgtggga gttgtagttg tattccaact 8401 tgtggccgag gatgtttccg tcctccttga aatcgattcc cttaagctcg atcctgttga 8461 cgagggtgtc tccctcaaac ttgacttcag cacgtgtctt gtagttcccg tcgtccttga 8521 agaagatggt cctctcctgc acgtatccct caggcatggc gctcttgaag aagtcgtgcc 8581 gcttcatatg atctgggtat cttgaaaagc attgaacacc ataagagaaa gtagtgacaa 8641 gtgttggcca gggaacaggt agttttccag tagtgcaaat aaatttaagg gtaagttttc 8701 cgtatgttgc atcaccttca ccctctccac tgacagaaaa tttgtgccca ttaacatcac 8761 catctaattc aacaagaatt gggacaactc cagtgaaaag ttctccttta cccatggcta 8821 tcgttcgtaa atggtgaaaa ttttcagaaa attgcttttg ctttaaaaga aatgatttaa 8881 attgctgcaa tagaagtaga atgcttgatt gcttgagatt cgtttgtttt gtatatgttg 8941 tgttgagaat tctcgaggtc ctctccaaat gaaatgaact tccttatata gaggaagggt 9001 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat 9061 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg 9121 ggggtccatc tttgggacca ctgtcggcag aggcatcttc aacgatggcc tttcctttat 9181 cgcaatgatg gcatttgtag gagccacctt ccttttccac tatcttcaca ataaagtgac 9241 agatagctgg gcaatggaat ccgaggaggt ttccggatat taccctttgt tgaaaagtct 9301 caattgccct ttggtcttct gagactgtat ctttgatatt tttggagtag acaagtgtgt 9361 cgtgctccac catgttatca catcaatcca cttgctttga agacgtggtt ggaacgtctt 9421 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg 9481 catcttcaac gatggccttt cctttatcgc aatgatggca tttgtaggag ccaccttcct 9541 tttccactat cttcacaata aagtgacaga tagctgggca atggaatccg aggaggtttc 9601 cggatattac cctttgttga aaagtctcaa ttgccctttg gtcttctgag actgtatctt 9661 tgatattttt ggagtagaca agtgtgtcgt gctccaccat gttgacctgc agg //