LOCUS       pt-yb        9853 bp    DNA   circular            18-OCT-2007
DEFINITION  pAN191.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|446572756|
COMMENT     VNTDBDATE|460547980|
COMMENT     VNTNAME|pt-yb|
COMMENT     VNTAUTHORNAME|NebenfŸhr Lab|
COMMENT     VNTAUTHORTEL|(865) 974-9201|
COMMENT     VNTAUTHORWWW|http://botany1.bio.utk.edu|
COMMENT     VNTAUTHORAD1|Univeristy of Tennessee|
COMMENT     VNTAUTHORAD2|Department of Botany|
COMMENT     VNTAUTHORAD3|1406 Circle Dr|
COMMENT     VNTAUTHORAD4|Knoxville, TN 37996-1100|
FEATURES             Location/Qualifiers
     repeat_region   8527..8553
                     /vntifkey="34"
                     /label=LB
                     /note="left border repeat from C58 T-DNA"
     polyA_signal    complement(8637..8888)
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="from mannopine synthase"
     gene            complement(8899..9450)
                     /gene="BAR"
                     /vntifkey="60"
     CDS             complement(8899..9450)
                     /gene="BAR"
                     /codon_start=1
                     /product="phosphinothricin acetyl transferase"
                     /protein_id="AAP78910.1"
                     /db_xref="GI:32265028"
                     /vntifkey="4"
                     /label=BAR-R
                     /note="basta resistance protein"
     promoter        complement(9456..9836)
                     /vntifkey="30"
                     /label=Promoter\(bar)
                     /note="mannopine synthase promoter"
     repeat_region   2249..2272
                     /vntifkey="34"
                     /label=RB
                     /note="right border repeat from C58 T-DNA"
     rep_origin      complement(3334..4334)
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="pVS1"
     rep_origin      complement(6737..7018)
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="pBR325"
     gene            complement(7308..8102)
                     /gene="aadA"
                     /vntifkey="60"
     CDS             complement(7308..8102)
                     /gene="aadA"
                     /function="kanamycin resistance"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAP78911.1"
                     /db_xref="GI:32265029"
                     /vntifkey="4"
                     /label=KAN-R
                     /note="aadA"
     primer_bind     672..695
                     /vntifkey="28"
                     /label=35S\primer
     promoter        10..736
                     /vntifkey="29"
                     /label=d35S
                     /note="double 35S promoter"
     terminator      1759..2008
                     /vntifkey="43"
                     /label=nos\3'
                     /note="terminator from nopaline synthase gene"
     CDS             776..1012
                     /vntifkey="4"
                     /label=cp\targeting\signal
                     /note="inserted as PCR product from pea RUBISCO small subunit"
     CDS             1019..1738
                     /vntifkey="4"
                     /label=EYFP
                     /note="EYFP coding sequence"
     primer_bind     complement(1072..1094)
                     /vntifkey="28"
                     /label=SEQ-GFP-NR
                     /note="annealing site of GFP sequencing primer"
BASE COUNT     2401 a      2730 c      2653 g      2069 t 
ORIGIN
        1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 
       61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 
      121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 
      181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 
      241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 
      301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 
      361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 
      421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 
      481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 
      541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 
      601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 
      661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 
      721 tcatttcatt tggagaggac agcccagatc aactagtctt aagtccggag ctagcatggc 
      781 ttcctcagtt ctttcctctg cagcagttgc cacccgcagc aatgttgctc aagctaacat 
      841 ggttgcacct ttcactggcc ttaagtcagc tgcctcattc cctgtttcaa ggaagcaaaa 
      901 ccttgacatc acttccattg ccagcaacgg cggaagagtg caatgcatgc aggtgtggcc 
      961 accaattaac aagaagaagt acgagactct ctcatacctt cctgatttga gcggatccat 
     1021 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg 
     1081 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg 
     1141 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct 
     1201 cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca 
     1261 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt 
     1321 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt 
     1381 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa 
     1441 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg 
     1501 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga 
     1561 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 
     1621 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct 
     1681 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag 
     1741 cggccgcccg ggctgcagtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt 
     1801 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat 
     1861 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt 
     1921 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg 
     1981 cgcggtgtca tctatgttac tagatcggga attcgatatc aagcttggca ctggccgtcg 
     2041 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 
     2101 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 
     2161 agttgcgcag cctgaatggc gaatgctaga gcagcttgag cttggatcag attgtcgttt 
     2221 cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag 
     2281 aaaagagcgt ttattagaat aacggatatt taaaagggcg tgaaaaggtt tatccgttcg 
     2341 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca 
     2401 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa 
     2461 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg 
     2521 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca 
     2581 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga 
     2641 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt 
     2701 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca 
     2761 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg 
     2821 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc 
     2881 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg 
     2941 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc 
     3001 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca 
     3061 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg 
     3121 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga 
     3181 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc 
     3241 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa 
     3301 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag 
     3361 ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc 
     3421 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa 
     3481 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga 
     3541 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga 
     3601 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg 
     3661 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg 
     3721 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg 
     3781 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact 
     3841 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt 
     3901 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg 
     3961 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg 
     4021 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc 
     4081 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg 
     4141 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt 
     4201 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc 
     4261 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa 
     4321 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg 
     4381 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat 
     4441 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc 
     4501 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc 
     4561 caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg 
     4621 aggaatcggc gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt 
     4681 gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca 
     4741 gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg 
     4801 caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca 
     4861 gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac 
     4921 gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag 
     4981 cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat 
     5041 tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa 
     5101 gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc 
     5161 tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta 
     5221 aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg 
     5281 gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg 
     5341 ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag 
     5401 aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt 
     5461 tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag 
     5521 acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc 
     5581 aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct 
     5641 ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc 
     5701 taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt 
     5761 ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac 
     5821 ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat 
     5881 ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt 
     5941 aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa 
     6001 gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg 
     6061 gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa 
     6121 gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt 
     6181 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 
     6241 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 
     6301 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat 
     6361 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga 
     6421 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg 
     6481 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 
     6541 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 
     6601 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 
     6661 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 
     6721 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 
     6781 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 
     6841 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 
     6901 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 
     6961 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 
     7021 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 
     7081 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 
     7141 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 
     7201 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 
     7261 tggaacgaaa actcacgtta agggattttg gtcatgcatt ctaggtacta aaacaattca 
     7321 tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa 
     7381 aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca 
     7441 atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg 
     7501 ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct 
     7561 tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct 
     7621 tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg 
     7681 gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag 
     7741 agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac 
     7801 tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc 
     7861 cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc 
     7921 ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat 
     7981 aggttttcat tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct 
     8041 tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc 
     8101 atttattatt tccttcctct tttctacagt atttaaagat accccaagaa gctaattata 
     8161 acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag 
     8221 ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga 
     8281 aaccgcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc 
     8341 gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg 
     8401 taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga 
     8461 tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct 
     8521 ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac 
     8581 attgcggacg tttttaatgt actgaattaa cgccgaatta attcgagctc ggatctgata 
     8641 atttatttga aaattcataa gaaaagcaaa cgttacatga attgatgaaa caatacaaag 
     8701 acagataaag ccacgcacat ttaggatatt ggccgagatt actgaatatt gagtaagatc 
     8761 acggaatttc tgacaggagc atgtcttcaa ttcagcccaa atggcagttg aaatactcaa 
     8821 accgccccat atgcaggagc ggatcattca ttgtttgttt ggttgccttt gccaacatgg 
     8881 gagtccaaga ttctgcagtt agatctcggt gacgggcagg accggacggg gcggtaccgg 
     8941 caggctgaag tccagctgcc agaaacccac gtcatgccag ttcccgtgct tgaagccggc 
     9001 cgcccgcagc atgccgcggg gggcatatcc gagcgcctcg tgcatgcgca cgctcgggtc 
     9061 gttgggcagc ccgatgacag cgaccacgct cttgaagccc tgtgcctcca gggacttcag 
     9121 caggtgggtg tagagcgtgg agcccagtcc cgtccgctgg tggcgggggg agacgtacac 
     9181 ggtcgactcg gccgtccagt cgtaggcgtt gcgtgccttc caggggcccg cgtaggcgat 
     9241 gccggcgacc tcgccgtcca cctcggcgac gagccaggga tagcgctccc gcagacggac 
     9301 gaggtcgtcc gtccactcct gcggttcctg cggctcggta cggaagttga ccgtgcttgt 
     9361 ctcgatgtag tggttgacga tggtgcagac cgccggcatg tccgcctcgg tggcacggcg 
     9421 gatgtcggcc gggcgtcgtt ctgggctcat cgattcgatt tggtgtatcg agattggtta 
     9481 tgaaattcag atgctagtgt aatgtattgg taatttggga agatataata ggaagcaagg 
     9541 ctatttatcc atttctgaaa aggcgaaatg gcgtcaccgc gagcgtcacg cgcattccgt 
     9601 tcttgctgta aagcgttgtt tggtacactt ttgactagcg aggcttggcg tgtcagcgta 
     9661 tctattcaaa agtcgttaat ggctgcggat caagaaaaag ttggaataga aacagaatac 
     9721 ccgcgaaatt caggcccggt tgccatgtcc tacacgccga aataaacgac caaattagta 
     9781 gaaaaataaa aactagctca gatacttacg tcacgtcttg cgcactgatt tgaaaaatct 
     9841 cagaattcga gct 
//
