LOCUS       pt-rb        9844 bp    DNA   circular            18-OCT-2007
DEFINITION  pAN612.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|443791263|
COMMENT     VNTDBDATE|460547848|
COMMENT     VNTNAME|pt-rb|
COMMENT     VNTAUTHORNAME|NebenfŸhr Lab|
COMMENT     VNTAUTHORTEL|(865) 974-9201|
COMMENT     VNTAUTHORWWW|http://botany1.bio.utk.edu|
COMMENT     VNTAUTHORAD1|Univeristy of Tennessee|
COMMENT     VNTAUTHORAD2|Department of Botany|
COMMENT     VNTAUTHORAD3|1406 Circle Dr|
COMMENT     VNTAUTHORAD4|Knoxville, TN 37996-1100|
FEATURES             Location/Qualifiers
     repeat_region   8518..8544
                     /vntifkey="34"
                     /label=LB
                     /note="left border repeat from C58 T-DNA"
     polyA_signal    complement(8628..8879)
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="from mannopine synthase"
     gene            complement(8890..9441)
                     /gene="BAR"
                     /vntifkey="60"
     CDS             complement(8890..9441)
                     /gene="BAR"
                     /codon_start=1
                     /product="phosphinothricin acetyl transferase"
                     /protein_id="AAP78910.1"
                     /db_xref="GI:32265028"
                     /vntifkey="4"
                     /label=BAR-R
                     /note="basta resistance protein"
     promoter        complement(9447..9827)
                     /vntifkey="30"
                     /label=Promoter\(bar)
                     /note="mannopine synthase promoter"
     repeat_region   2240..2263
                     /vntifkey="34"
                     /label=RB
                     /note="right border repeat from C58 T-DNA"
     rep_origin      complement(3325..4325)
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="pVS1"
     rep_origin      complement(6728..7009)
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="pBR325"
     gene            complement(7299..8093)
                     /gene="aadA"
                     /vntifkey="60"
     CDS             complement(7299..8093)
                     /gene="aadA"
                     /function="kanamycin resistance"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAP78911.1"
                     /db_xref="GI:32265029"
                     /vntifkey="4"
                     /label=KAN-R
                     /note="aadA"
     CDS             1019..1729
                     /vntifkey="4"
                     /label=mCherry
     primer_bind     672..695
                     /vntifkey="28"
                     /label=35S\primer
     promoter        10..736
                     /vntifkey="29"
                     /label=d35S
                     /note="double 35S promoter"
     terminator      1750..1999
                     /vntifkey="43"
                     /label=nos\3'
                     /note="terminator from nopaline synthase gene"
     CDS             776..1012
                     /vntifkey="4"
                     /label=cp\targeting\signal
                     /note="inserted as PCR product from pea RUBISCO small subunit"
BASE COUNT     2394 a      2715 c      2670 g      2065 t 
ORIGIN
        1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 
       61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 
      121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 
      181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 
      241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 
      301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 
      361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 
      421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 
      481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 
      541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 
      601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 
      661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 
      721 tcatttcatt tggagaggac agcccagatc aactagtctt aagtccggag ctagcatggc 
      781 ttcctcagtt ctttcctctg cagcagttgc cacccgcagc aatgttgctc aagctaacat 
      841 ggttgcacct ttcactggcc ttaagtcagc tgcctcattc cctgtttcaa ggaagcaaaa 
      901 ccttgacatc acttccattg ccagcaacgg cggaagagtg caatgcatgc aggtgtggcc 
      961 accaattaac aagaagaagt acgagactct ctcatacctt cctgatttga gcggatccat 
     1021 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt 
     1081 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg 
     1141 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt 
     1201 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc 
     1261 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt 
     1321 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg 
     1381 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat 
     1441 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc 
     1501 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga 
     1561 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa 
     1621 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg 
     1681 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaaa gcggccgccc 
     1741 gggctgcagt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct 
     1801 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta 
     1861 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta 
     1921 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc 
     1981 atctatgtta ctagatcggg aattcgatat caagcttggc actggccgtc gttttacaac 
     2041 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 
     2101 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca 
     2161 gcctgaatgg cgaatgctag agcagcttga gcttggatca gattgtcgtt tcccgccttc 
     2221 agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg 
     2281 tttattagaa taacggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt 
     2341 atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg 
     2401 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc 
     2461 gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt 
     2521 cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat 
     2581 gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga 
     2641 cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa 
     2701 gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc 
     2761 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact 
     2821 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg 
     2881 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga 
     2941 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg 
     3001 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga 
     3061 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg 
     3121 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg 
     3181 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa 
     3241 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc 
     3301 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg 
     3361 cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg 
     3421 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt 
     3481 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat 
     3541 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt 
     3601 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg 
     3661 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag 
     3721 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca 
     3781 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt 
     3841 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat 
     3901 gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc 
     3961 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg 
     4021 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga 
     4081 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg 
     4141 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg 
     4201 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg 
     4261 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa 
     4321 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa 
     4381 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg 
     4441 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac 
     4501 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa 
     4561 gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg 
     4621 cgtgacggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg 
     4681 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc 
     4741 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg 
     4801 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc 
     4861 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt 
     4921 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac 
     4981 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg 
     5041 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga 
     5101 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga 
     5161 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg 
     5221 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag 
     5281 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac 
     5341 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac 
     5401 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac 
     5461 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac 
     5521 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc 
     5581 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc 
     5641 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg 
     5701 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct 
     5761 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt 
     5821 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag 
     5881 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc 
     5941 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc 
     6001 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc 
     6061 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg 
     6121 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga 
     6181 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 
     6241 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc 
     6301 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca 
     6361 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg 
     6421 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 
     6481 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 
     6541 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
     6601 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
     6661 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
     6721 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
     6781 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 
     6841 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
     6901 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
     6961 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
     7021 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 
     7081 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
     7141 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
     7201 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
     7261 aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa 
     7321 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg 
     7381 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac 
     7441 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc 
     7501 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt 
     7561 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag 
     7621 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg 
     7681 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg 
     7741 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag 
     7801 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag 
     7861 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt 
     7921 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca 
     7981 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag 
     8041 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat 
     8101 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga 
     8161 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa 
     8221 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt 
     8281 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca 
     8341 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag 
     8401 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc 
     8461 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg 
     8521 caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac 
     8581 gtttttaatg tactgaatta acgccgaatt aattcgagct cggatctgat aatttatttg 
     8641 aaaattcata agaaaagcaa acgttacatg aattgatgaa acaatacaaa gacagataaa 
     8701 gccacgcaca tttaggatat tggccgagat tactgaatat tgagtaagat cacggaattt 
     8761 ctgacaggag catgtcttca attcagccca aatggcagtt gaaatactca aaccgcccca 
     8821 tatgcaggag cggatcattc attgtttgtt tggttgcctt tgccaacatg ggagtccaag 
     8881 attctgcagt tagatctcgg tgacgggcag gaccggacgg ggcggtaccg gcaggctgaa 
     8941 gtccagctgc cagaaaccca cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag 
     9001 catgccgcgg ggggcatatc cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag 
     9061 cccgatgaca gcgaccacgc tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt 
     9121 gtagagcgtg gagcccagtc ccgtccgctg gtggcggggg gagacgtaca cggtcgactc 
     9181 ggccgtccag tcgtaggcgt tgcgtgcctt ccaggggccc gcgtaggcga tgccggcgac 
     9241 ctcgccgtcc acctcggcga cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc 
     9301 cgtccactcc tgcggttcct gcggctcggt acggaagttg accgtgcttg tctcgatgta 
     9361 gtggttgacg atggtgcaga ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc 
     9421 cgggcgtcgt tctgggctca tcgattcgat ttggtgtatc gagattggtt atgaaattca 
     9481 gatgctagtg taatgtattg gtaatttggg aagatataat aggaagcaag gctatttatc 
     9541 catttctgaa aaggcgaaat ggcgtcaccg cgagcgtcac gcgcattccg ttcttgctgt 
     9601 aaagcgttgt ttggtacact tttgactagc gaggcttggc gtgtcagcgt atctattcaa 
     9661 aagtcgttaa tggctgcgga tcaagaaaaa gttggaatag aaacagaata cccgcgaaat 
     9721 tcaggcccgg ttgccatgtc ctacacgccg aaataaacga ccaaattagt agaaaaataa 
     9781 aaactagctc agatacttac gtcacgtctt gcgcactgat ttgaaaaatc tcagaattcg 
     9841 agct 
//
