LOCUS       pm-yb       10485 bp    DNA   circular            18-OCT-2007
DEFINITION  pAN622.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|447858940|
COMMENT     VNTDBDATE|460547598|
COMMENT     VNTNAME|pm-yb|
COMMENT     VNTAUTHORNAME|NebenfŸhr Lab|
COMMENT     VNTAUTHORTEL|(865) 974-9201|
COMMENT     VNTAUTHORWWW|http://botany1.bio.utk.edu|
COMMENT     VNTAUTHORAD1|Univeristy of Tennessee|
COMMENT     VNTAUTHORAD2|Department of Botany|
COMMENT     VNTAUTHORAD3|1406 Circle Dr|
COMMENT     VNTAUTHORAD4|Knoxville, TN 37996-1100|
FEATURES             Location/Qualifiers
     repeat_region   9159..9185
                     /vntifkey="34"
                     /label=LB
                     /note="left border repeat from C58 T-DNA"
     polyA_signal    complement(9269..9520)
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="from mannopine synthase"
     gene            complement(9531..10082)
                     /gene="BAR"
                     /vntifkey="60"
     CDS             complement(9531..10082)
                     /gene="BAR"
                     /codon_start=1
                     /product="phosphinothricin acetyl transferase"
                     /protein_id="AAP78910.1"
                     /db_xref="GI:32265028"
                     /vntifkey="4"
                     /label=BAR-R
                     /note="basta resistance protein"
     promoter        complement(10088..10468)
                     /vntifkey="30"
                     /label=Promoter\(bar)
                     /note="mannopine synthase promoter"
     repeat_region   2881..2904
                     /vntifkey="34"
                     /label=RB
                     /note="right border repeat from C58 T-DNA"
     rep_origin      complement(3966..4966)
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="pVS1"
     rep_origin      complement(7369..7650)
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="pBR325"
     gene            complement(7940..8734)
                     /gene="aadA"
                     /vntifkey="60"
     CDS             complement(7940..8734)
                     /gene="aadA"
                     /function="kanamycin resistance"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAP78911.1"
                     /db_xref="GI:32265029"
                     /vntifkey="4"
                     /label=KAN-R
                     /note="aadA"
     CDS             782..1643
                     /vntifkey="4"
                     /label=PIP2A
     promoter        10..736
                     /vntifkey="29"
                     /label=d35S
                     /note="double 35S promoter"
     terminator      2391..2640
                     /vntifkey="43"
                     /label=nos\3'
                     /note="terminator from nopaline synthase gene"
     primer_bind     672..695
                     /vntifkey="28"
                     /label=35S\primer
     CDS             1649..2371
                     /vntifkey="4"
                     /label=EYFP
                     /note="EYFP coding sequence"
     primer_bind     complement(1702..1724)
                     /vntifkey="28"
                     /label=SEQ-GFP-NR
                     /note="annealing site of GFP sequencing primer"
     primer_bind     2240..2259
                     /vntifkey="28"
                     /label=SEQ-YFP-F1
                     /note="primer is specific for YFP"
     primer_bind     complement(1844..1861)
                     /vntifkey="28"
                     /label=SEQ-YFP-R1
                     /note="primer is specific for YFP"
     primer_bind     complement(2357..2373)
                     /vntifkey="28"
                     /label=YFP-R
BASE COUNT     2534 a      2879 c      2820 g      2252 t 
ORIGIN
        1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 
       61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 
      121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 
      181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 
      241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 
      301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 
      361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 
      421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 
      481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 
      541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 
      601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 
      661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 
      721 tcatttcatt tggagaggac agcccagatc aactagtctt aagtccggag ctagctctag 
      781 aatggcaaag gatgtggaag ccgttcccgg agaaggattt cagacaagag actatcaaga 
      841 tccgccacca gctccgttta ttgatggagc ggagctaaag aagtggtctt tctacagagc 
      901 agttatcgcc gagttcgtag ccactctcct cttcttatac atcaccgttt tgacagtcat 
      961 cggttacaag attcagtccg atactgatgc cggtggagta gattgcggcg gagttggaat 
     1021 cctcggtatc gcttgggcct ttggtggtat gatcttcatc ctcgtctact gcaccgccgg 
     1081 tatctctggt ggtcacatta acccagcggt gacatttggg ctattcttgg cacgtaaagt 
     1141 gtcgttacct agggccctat tgtacataat cgctcagtgt ttgggtgcga tttgtggagt 
     1201 tggttttgtc aaagccttcc aaagctctta ctacacccgt tacggaggtg gagccaactc 
     1261 tctagccgat ggctacagca cagggaccgg tttagccgca gagatcattg gtactttcgt 
     1321 tcttgtctac actgtcttct ctgctactga ccccaaacgt agtgctagag actcccacgt 
     1381 tccggtgttg gcgccacttc caatcggatt tgccgtgttc atggtacatt tggctaccat 
     1441 tcccattacc ggaaccggaa ttaacccggc aaggagtttc ggagctgccg taatctacaa 
     1501 caagagcaag ccatgggatg accactggat attttgggtt ggaccattca ttggagctgc 
     1561 gatagctgca ttctaccacc aattcgttct gagagcttca ggttctaagt ctctcggatc 
     1621 attcagaagt gctgccaacg tcggatccat ggtgagcaag ggcgaggagc tgttcaccgg 
     1681 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc 
     1741 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac 
     1801 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctacagtg 
     1861 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga 
     1921 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc 
     1981 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt 
     2041 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt 
     2101 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa 
     2161 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga 
     2221 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga 
     2281 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac 
     2341 tctcggcatg gacgagctgt acagatctta aagcggccgc ccggctgcag ttcaaacatt 
     2401 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa 
     2461 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg 
     2521 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa 
     2581 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg 
     2641 gaattcgata tcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 
     2701 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 
     2761 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatgcta 
     2821 gagcagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt 
     2881 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataacggata 
     2941 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag 
     3001 ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc 
     3061 ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg 
     3121 cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata 
     3181 aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct 
     3241 ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc 
     3301 gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc 
     3361 gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg 
     3421 accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc 
     3481 caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg 
     3541 gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc 
     3601 atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc 
     3661 cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc 
     3721 cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca 
     3781 cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag 
     3841 gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa 
     3901 gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg 
     3961 cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca accgtgcggc 
     4021 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg 
     4081 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct 
     4141 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga 
     4201 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc 
     4261 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga 
     4321 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt 
     4381 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt 
     4441 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga 
     4501 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt 
     4561 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt 
     4621 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta 
     4681 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc 
     4741 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc 
     4801 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc 
     4861 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg 
     4921 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag 
     4981 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta 
     5041 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat 
     5101 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga 
     5161 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg 
     5221 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgacggt cgcaaaccat 
     5281 ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg 
     5341 cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag 
     5401 cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga 
     5461 ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg 
     5521 tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg 
     5581 accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg 
     5641 cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc 
     5701 atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt 
     5761 tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga 
     5821 aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta 
     5881 cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc 
     5941 gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg 
     6001 attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg 
     6061 attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg 
     6121 caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg 
     6181 gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg 
     6241 agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca 
     6301 acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa 
     6361 ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg 
     6421 ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca 
     6481 ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg 
     6541 cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt 
     6601 ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc 
     6661 tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc 
     6721 tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc 
     6781 gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac 
     6841 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc 
     6901 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt 
     6961 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag 
     7021 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 
     7081 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 
     7141 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 
     7201 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 
     7261 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 
     7321 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 
     7381 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 
     7441 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 
     7501 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 
     7561 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 
     7621 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 
     7681 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 
     7741 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 
     7801 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 
     7861 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 
     7921 tggtcatgca ttctaggtac taaaacaatt catccagtaa aatataatat tttattttct 
     7981 cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga 
     8041 tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc 
     8101 ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc 
     8161 ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat 
     8221 acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg 
     8281 ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat 
     8341 agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga 
     8401 taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct 
     8461 cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag 
     8521 gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc 
     8581 ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat 
     8641 ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt 
     8701 ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca 
     8761 gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct 
     8821 tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc 
     8881 gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg cagcaacgct 
     8941 ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc 
     9001 agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca 
     9061 acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt 
     9121 gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta 
     9181 aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat gtactgaatt 
     9241 aacgccgaat taattcgagc tcggatctga taatttattt gaaaattcat aagaaaagca 
     9301 aacgttacat gaattgatga aacaatacaa agacagataa agccacgcac atttaggata 
     9361 ttggccgaga ttactgaata ttgagtaaga tcacggaatt tctgacagga gcatgtcttc 
     9421 aattcagccc aaatggcagt tgaaatactc aaaccgcccc atatgcagga gcggatcatt 
     9481 cattgtttgt ttggttgcct ttgccaacat gggagtccaa gattctgcag ttagatctcg 
     9541 gtgacgggca ggaccggacg gggcggtacc ggcaggctga agtccagctg ccagaaaccc 
     9601 acgtcatgcc agttcccgtg cttgaagccg gccgcccgca gcatgccgcg gggggcatat 
     9661 ccgagcgcct cgtgcatgcg cacgctcggg tcgttgggca gcccgatgac agcgaccacg 
     9721 ctcttgaagc cctgtgcctc cagggacttc agcaggtggg tgtagagcgt ggagcccagt 
     9781 cccgtccgct ggtggcgggg ggagacgtac acggtcgact cggccgtcca gtcgtaggcg 
     9841 ttgcgtgcct tccaggggcc cgcgtaggcg atgccggcga cctcgccgtc cacctcggcg 
     9901 acgagccagg gatagcgctc ccgcagacgg acgaggtcgt ccgtccactc ctgcggttcc 
     9961 tgcggctcgg tacggaagtt gaccgtgctt gtctcgatgt agtggttgac gatggtgcag 
    10021 accgccggca tgtccgcctc ggtggcacgg cggatgtcgg ccgggcgtcg ttctgggctc 
    10081 atcgattcga tttggtgtat cgagattggt tatgaaattc agatgctagt gtaatgtatt 
    10141 ggtaatttgg gaagatataa taggaagcaa ggctatttat ccatttctga aaaggcgaaa 
    10201 tggcgtcacc gcgagcgtca cgcgcattcc gttcttgctg taaagcgttg tttggtacac 
    10261 ttttgactag cgaggcttgg cgtgtcagcg tatctattca aaagtcgtta atggctgcgg 
    10321 atcaagaaaa agttggaata gaaacagaat acccgcgaaa ttcaggcccg gttgccatgt 
    10381 cctacacgcc gaaataaacg accaaattag tagaaaaata aaaactagct cagatactta 
    10441 cgtcacgtct tgcgcactga tttgaaaaat ctcagaattc gagct 
//
