LOCUS       pm-rb       10476 bp    DNA   circular            18-OCT-2007
DEFINITION  pAN610.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|443794193|
COMMENT     VNTDBDATE|460547560|
COMMENT     VNTNAME|pm-rb|
COMMENT     VNTAUTHORNAME|NebenfŸhr Lab|
COMMENT     VNTAUTHORTEL|(865) 974-9201|
COMMENT     VNTAUTHORWWW|http://botany1.bio.utk.edu|
COMMENT     VNTAUTHORAD1|Univeristy of Tennessee|
COMMENT     VNTAUTHORAD2|Department of Botany|
COMMENT     VNTAUTHORAD3|1406 Circle Dr|
COMMENT     VNTAUTHORAD4|Knoxville, TN 37996-1100|
FEATURES             Location/Qualifiers
     repeat_region   9150..9176
                     /vntifkey="34"
                     /label=LB
                     /note="left border repeat from C58 T-DNA"
     polyA_signal    complement(9260..9511)
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="from mannopine synthase"
     gene            complement(9522..10073)
                     /gene="BAR"
                     /vntifkey="60"
     CDS             complement(9522..10073)
                     /gene="BAR"
                     /codon_start=1
                     /product="phosphinothricin acetyl transferase"
                     /protein_id="AAP78910.1"
                     /db_xref="GI:32265028"
                     /vntifkey="4"
                     /label=BAR-R
                     /note="basta resistance protein"
     promoter        complement(10079..10459)
                     /vntifkey="30"
                     /label=Promoter\(bar)
                     /note="mannopine synthase promoter"
     repeat_region   2872..2895
                     /vntifkey="34"
                     /label=RB
                     /note="right border repeat from C58 T-DNA"
     rep_origin      complement(3957..4957)
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="pVS1"
     rep_origin      complement(7360..7641)
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="pBR325"
     gene            complement(7931..8725)
                     /gene="aadA"
                     /vntifkey="60"
     CDS             complement(7931..8725)
                     /gene="aadA"
                     /function="kanamycin resistance"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAP78911.1"
                     /db_xref="GI:32265029"
                     /vntifkey="4"
                     /label=KAN-R
                     /note="aadA"
     CDS             1649..2362
                     /vntifkey="4"
                     /label=mCherry
     promoter        10..736
                     /vntifkey="29"
                     /label=d35S
                     /note="double 35S promoter"
     terminator      2382..2631
                     /vntifkey="43"
                     /label=nos\3'
                     /note="terminator from nopaline synthase gene"
     primer_bind     672..695
                     /vntifkey="28"
                     /label=35S\primer
     CDS             782..1643
                     /vntifkey="4"
                     /label=PIP2A
BASE COUNT     2526 a      2864 c      2838 g      2248 t 
ORIGIN
        1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 
       61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 
      121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 
      181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 
      241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 
      301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 
      361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 
      421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 
      481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 
      541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 
      601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 
      661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 
      721 tcatttcatt tggagaggac agcccagatc aactagtctt aagtccggag ctagctctag 
      781 aatggcaaag gatgtggaag ccgttcccgg agaaggattt cagacaagag actatcaaga 
      841 tccgccacca gctccgttta ttgatggagc ggagctaaag aagtggtctt tctacagagc 
      901 agttatcgcc gagttcgtag ccactctcct cttcttatac atcaccgttt tgacagtcat 
      961 cggttacaag attcagtccg atactgatgc cggtggagta gattgcggcg gagttggaat 
     1021 cctcggtatc gcttgggcct ttggtggtat gatcttcatc ctcgtctact gcaccgccgg 
     1081 tatctctggt ggtcacatta acccagcggt gacatttggg ctattcttgg cacgtaaagt 
     1141 gtcgttacct agggccctat tgtacataat cgctcagtgt ttgggtgcga tttgtggagt 
     1201 tggttttgtc aaagccttcc aaagctctta ctacacccgt tacggaggtg gagccaactc 
     1261 tctagccgat ggctacagca cagggaccgg tttagccgca gagatcattg gtactttcgt 
     1321 tcttgtctac actgtcttct ctgctactga ccccaaacgt agtgctagag actcccacgt 
     1381 tccggtgttg gcgccacttc caatcggatt tgccgtgttc atggtacatt tggctaccat 
     1441 tcccattacc ggaaccggaa ttaacccggc aaggagtttc ggagctgccg taatctacaa 
     1501 caagagcaag ccatgggatg accactggat attttgggtt ggaccattca ttggagctgc 
     1561 gatagctgca ttctaccacc aattcgttct gagagcttca ggttctaagt ctctcggatc 
     1621 attcagaagt gctgccaacg tcggatccat ggtgagcaag ggcgaggagg ataacatggc 
     1681 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga 
     1741 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct 
     1801 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat 
     1861 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc 
     1921 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac 
     1981 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg 
     2041 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc 
     2101 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa 
     2161 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc 
     2221 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga 
     2281 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat 
     2341 ggacgagctg tacagatctt aaagcggccg cccggctgca gttcaaacat ttggcaataa 
     2401 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg 
     2461 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt 
     2521 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc 
     2581 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattcgat 
     2641 atcaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac 
     2701 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc 
     2761 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgct agagcagctt 
     2821 gagcttggat cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat 
     2881 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataacggat atttaaaagg 
     2941 gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct 
     3001 cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt 
     3061 tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct 
     3121 gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat 
     3181 acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg 
     3241 ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac 
     3301 gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg 
     3361 gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta 
     3421 gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc 
     3481 ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc 
     3541 atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc 
     3601 acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc 
     3661 ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg 
     3721 aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc 
     3781 agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg 
     3841 accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac 
     3901 cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga 
     3961 tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa 
     4021 tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag 
     4081 aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg 
     4141 cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa 
     4201 ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct 
     4261 agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg 
     4321 cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga 
     4381 ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga 
     4441 cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct 
     4501 gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt 
     4561 taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc 
     4621 gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc 
     4681 cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac 
     4741 aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc 
     4801 tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca 
     4861 aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc 
     4921 ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc 
     4981 aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac 
     5041 atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc 
     5101 taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat 
     5161 gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa 
     5221 tggcactgga acccccaagc ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg 
     5281 tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg 
     5341 cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg 
     5401 atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc 
     5461 cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc 
     5521 gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag 
     5581 ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg 
     5641 ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg 
     5701 aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac 
     5761 acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc 
     5821 tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg 
     5881 ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga 
     5941 tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt 
     6001 accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt 
     6061 tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg 
     6121 cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca 
     6181 agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt 
     6241 tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg 
     6301 agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag 
     6361 caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa 
     6421 agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc 
     6481 ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact 
     6541 ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc 
     6601 gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg 
     6661 ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag 
     6721 gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc 
     6781 aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc 
     6841 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc 
     6901 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc 
     6961 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata 
     7021 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg 
     7081 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 
     7141 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 
     7201 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 
     7261 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 
     7321 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 
     7381 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 
     7441 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 
     7501 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 
     7561 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 
     7621 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 
     7681 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 
     7741 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 
     7801 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 
     7861 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc 
     7921 attctaggta ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag 
     7981 gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc 
     8041 tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa 
     8101 gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc 
     8161 cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc 
     8221 gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt 
     8281 tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat 
     8341 ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt 
     8401 cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga 
     8461 gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc 
     8521 cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc 
     8581 ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag 
     8641 caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt 
     8701 ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa 
     8761 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta 
     8821 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat 
     8881 agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg 
     8941 ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt 
     9001 gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc 
     9061 ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc 
     9121 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg 
     9181 acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa 
     9241 ttaattcgag ctcggatctg ataatttatt tgaaaattca taagaaaagc aaacgttaca 
     9301 tgaattgatg aaacaataca aagacagata aagccacgca catttaggat attggccgag 
     9361 attactgaat attgagtaag atcacggaat ttctgacagg agcatgtctt caattcagcc 
     9421 caaatggcag ttgaaatact caaaccgccc catatgcagg agcggatcat tcattgtttg 
     9481 tttggttgcc tttgccaaca tgggagtcca agattctgca gttagatctc ggtgacgggc 
     9541 aggaccggac ggggcggtac cggcaggctg aagtccagct gccagaaacc cacgtcatgc 
     9601 cagttcccgt gcttgaagcc ggccgcccgc agcatgccgc ggggggcata tccgagcgcc 
     9661 tcgtgcatgc gcacgctcgg gtcgttgggc agcccgatga cagcgaccac gctcttgaag 
     9721 ccctgtgcct ccagggactt cagcaggtgg gtgtagagcg tggagcccag tcccgtccgc 
     9781 tggtggcggg gggagacgta cacggtcgac tcggccgtcc agtcgtaggc gttgcgtgcc 
     9841 ttccaggggc ccgcgtaggc gatgccggcg acctcgccgt ccacctcggc gacgagccag 
     9901 ggatagcgct cccgcagacg gacgaggtcg tccgtccact cctgcggttc ctgcggctcg 
     9961 gtacggaagt tgaccgtgct tgtctcgatg tagtggttga cgatggtgca gaccgccggc 
    10021 atgtccgcct cggtggcacg gcggatgtcg gccgggcgtc gttctgggct catcgattcg 
    10081 atttggtgta tcgagattgg ttatgaaatt cagatgctag tgtaatgtat tggtaatttg 
    10141 ggaagatata ataggaagca aggctattta tccatttctg aaaaggcgaa atggcgtcac 
    10201 cgcgagcgtc acgcgcattc cgttcttgct gtaaagcgtt gtttggtaca cttttgacta 
    10261 gcgaggcttg gcgtgtcagc gtatctattc aaaagtcgtt aatggctgcg gatcaagaaa 
    10321 aagttggaat agaaacagaa tacccgcgaa attcaggccc ggttgccatg tcctacacgc 
    10381 cgaaataaac gaccaaatta gtagaaaaat aaaaactagc tcagatactt acgtcacgtc 
    10441 ttgcgcactg atttgaaaaa tctcagaatt cgagct 
//
