LOCUS pm-gb 10485 bp DNA circular 18-OCT-2007 DEFINITION pAN624. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|447263913| COMMENT VNTDBDATE|460547450| COMMENT VNTNAME|pm-gb| COMMENT VNTAUTHORNAME|NebenfŸhr Lab| COMMENT VNTAUTHORTEL|(865) 974-9201| COMMENT VNTAUTHORWWW|http://botany1.bio.utk.edu| COMMENT VNTAUTHORAD1|Univeristy of Tennessee| COMMENT VNTAUTHORAD2|Department of Botany| COMMENT VNTAUTHORAD3|1406 Circle Dr| COMMENT VNTAUTHORAD4|Knoxville, TN 37996-1100| FEATURES Location/Qualifiers repeat_region 9159..9185 /vntifkey="34" /label=LB /note="left border repeat from C58 T-DNA" polyA_signal complement(9269..9520) /vntifkey="25" /label=PolyA_Signal_1 /note="from mannopine synthase" gene complement(9531..10082) /gene="BAR" /vntifkey="60" CDS complement(9531..10082) /gene="BAR" /codon_start=1 /product="phosphinothricin acetyl transferase" /protein_id="AAP78910.1" /db_xref="GI:32265028" /vntifkey="4" /label=BAR-R /note="basta resistance protein" promoter complement(10088..10468) /vntifkey="30" /label=Promoter\(bar) /note="mannopine synthase promoter" repeat_region 2881..2904 /vntifkey="34" /label=RB /note="right border repeat from C58 T-DNA" rep_origin complement(3966..4966) /vntifkey="33" /label=Rep_Origin_1 /note="pVS1" rep_origin complement(7369..7650) /vntifkey="33" /label=Rep_Origin_2 /note="pBR325" gene complement(7940..8734) /gene="aadA" /vntifkey="60" CDS complement(7940..8734) /gene="aadA" /function="kanamycin resistance" /codon_start=1 /product="aminoglycoside phosphotransferase" /protein_id="AAP78911.1" /db_xref="GI:32265029" /vntifkey="4" /label=KAN-R /note="aadA" promoter 10..736 /vntifkey="29" /label=d35S /note="double 35S promoter" terminator 2391..2640 /vntifkey="43" /label=nos\3' /note="terminator from nopaline synthase gene" primer_bind 672..695 /vntifkey="28" /label=35S\primer CDS 782..1643 /vntifkey="4" /label=PIP2A CDS 1649..2371 /vntifkey="4" /label=GFP primer_bind complement(1703..1725) /vntifkey="28" /label=GFP-R\primer primer_bind 2333..2352 /vntifkey="28" /label=XFP-C-F\primer /note="sequencing primer" primer_bind 2299..2318 /vntifkey="28" /label=XFP-C-F2\primer /note="sequencing primer" BASE COUNT 2535 a 2879 c 2821 g 2250 t ORIGIN 1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 721 tcatttcatt tggagaggac agcccagatc aactagtctt aagtccggag ctagctctag 781 aatggcaaag gatgtggaag ccgttcccgg agaaggattt cagacaagag actatcaaga 841 tccgccacca gctccgttta ttgatggagc ggagctaaag aagtggtctt tctacagagc 901 agttatcgcc gagttcgtag ccactctcct cttcttatac atcaccgttt tgacagtcat 961 cggttacaag attcagtccg atactgatgc cggtggagta gattgcggcg gagttggaat 1021 cctcggtatc gcttgggcct ttggtggtat gatcttcatc ctcgtctact gcaccgccgg 1081 tatctctggt ggtcacatta acccagcggt gacatttggg ctattcttgg cacgtaaagt 1141 gtcgttacct agggccctat tgtacataat cgctcagtgt ttgggtgcga tttgtggagt 1201 tggttttgtc aaagccttcc aaagctctta ctacacccgt tacggaggtg gagccaactc 1261 tctagccgat ggctacagca cagggaccgg tttagccgca gagatcattg gtactttcgt 1321 tcttgtctac actgtcttct ctgctactga ccccaaacgt agtgctagag actcccacgt 1381 tccggtgttg gcgccacttc caatcggatt tgccgtgttc atggtacatt tggctaccat 1441 tcccattacc ggaaccggaa ttaacccggc aaggagtttc ggagctgccg taatctacaa 1501 caagagcaag ccatgggatg accactggat attttgggtt ggaccattca ttggagctgc 1561 gatagctgca ttctaccacc aattcgttct gagagcttca ggttctaagt ctctcggatc 1621 attcagaagt gctgccaacg tcggatccat ggtgagcaag ggcgaggagc tgttcaccgg 1681 ggtggtgccc atcctggtcg agctggacgg cgacgtgaac ggccacaagt tcagcgtgtc 1741 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac 1801 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcacctacg gcgtgcagtg 1861 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga 1921 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc 1981 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt 2041 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt 2101 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa 2161 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga 2221 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga 2281 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac 2341 tcacggcatg gacgagctgt acagatctta aagcggccgc ccggctgcag ttcaaacatt 2401 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa 2461 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg 2521 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa 2581 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg 2641 gaattcgata tcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 2701 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 2761 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatgcta 2821 gagcagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt 2881 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataacggata 2941 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag 3001 ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc 3061 ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg 3121 cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata 3181 aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct 3241 ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc 3301 gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc 3361 gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg 3421 accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc 3481 caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg 3541 gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc 3601 atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc 3661 cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc 3721 cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca 3781 cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag 3841 gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa 3901 gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg 3961 cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca accgtgcggc 4021 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg 4081 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct 4141 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga 4201 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc 4261 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga 4321 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt 4381 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt 4441 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga 4501 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt 4561 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt 4621 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta 4681 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc 4741 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc 4801 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc 4861 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg 4921 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag 4981 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta 5041 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat 5101 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga 5161 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg 5221 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgacggt cgcaaaccat 5281 ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg 5341 cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag 5401 cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga 5461 ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg 5521 tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg 5581 accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg 5641 cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc 5701 atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt 5761 tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga 5821 aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta 5881 cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc 5941 gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg 6001 attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg 6061 attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg 6121 caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg 6181 gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg 6241 agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca 6301 acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa 6361 ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg 6421 ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca 6481 ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg 6541 cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt 6601 ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc 6661 tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc 6721 tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc 6781 gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac 6841 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc 6901 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt 6961 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag 7021 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 7081 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 7141 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 7201 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 7261 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 7321 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 7381 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 7441 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 7501 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 7561 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 7621 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 7681 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 7741 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 7801 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 7861 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 7921 tggtcatgca ttctaggtac taaaacaatt catccagtaa aatataatat tttattttct 7981 cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga 8041 tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc 8101 ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc 8161 ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat 8221 acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg 8281 ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat 8341 agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga 8401 taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct 8461 cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag 8521 gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc 8581 ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat 8641 ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt 8701 ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca 8761 gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct 8821 tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc 8881 gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg cagcaacgct 8941 ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc 9001 agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca 9061 acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt 9121 gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta 9181 aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat gtactgaatt 9241 aacgccgaat taattcgagc tcggatctga taatttattt gaaaattcat aagaaaagca 9301 aacgttacat gaattgatga aacaatacaa agacagataa agccacgcac atttaggata 9361 ttggccgaga ttactgaata ttgagtaaga tcacggaatt tctgacagga gcatgtcttc 9421 aattcagccc aaatggcagt tgaaatactc aaaccgcccc atatgcagga gcggatcatt 9481 cattgtttgt ttggttgcct ttgccaacat gggagtccaa gattctgcag ttagatctcg 9541 gtgacgggca ggaccggacg gggcggtacc ggcaggctga agtccagctg ccagaaaccc 9601 acgtcatgcc agttcccgtg cttgaagccg gccgcccgca gcatgccgcg gggggcatat 9661 ccgagcgcct cgtgcatgcg cacgctcggg tcgttgggca gcccgatgac agcgaccacg 9721 ctcttgaagc cctgtgcctc cagggacttc agcaggtggg tgtagagcgt ggagcccagt 9781 cccgtccgct ggtggcgggg ggagacgtac acggtcgact cggccgtcca gtcgtaggcg 9841 ttgcgtgcct tccaggggcc cgcgtaggcg atgccggcga cctcgccgtc cacctcggcg 9901 acgagccagg gatagcgctc ccgcagacgg acgaggtcgt ccgtccactc ctgcggttcc 9961 tgcggctcgg tacggaagtt gaccgtgctt gtctcgatgt agtggttgac gatggtgcag 10021 accgccggca tgtccgcctc ggtggcacgg cggatgtcgg ccgggcgtcg ttctgggctc 10081 atcgattcga tttggtgtat cgagattggt tatgaaattc agatgctagt gtaatgtatt 10141 ggtaatttgg gaagatataa taggaagcaa ggctatttat ccatttctga aaaggcgaaa 10201 tggcgtcacc gcgagcgtca cgcgcattcc gttcttgctg taaagcgttg tttggtacac 10261 ttttgactag cgaggcttgg cgtgtcagcg tatctattca aaagtcgtta atggctgcgg 10321 atcaagaaaa agttggaata gaaacagaat acccgcgaaa ttcaggcccg gttgccatgt 10381 cctacacgcc gaaataaacg accaaattag tagaaaaata aaaactagct cagatactta 10441 cgtcacgtct tgcgcactga tttgaaaaat ctcagaattc gagct //