LOCUS       pm-cb       10482 bp    DNA   circular            18-OCT-2007
DEFINITION  pAN623.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|447858999|
COMMENT     VNTDBDATE|460547385|
COMMENT     VNTNAME|pm-cb|
COMMENT     VNTAUTHORNAME|NebenfŸhr Lab|
COMMENT     VNTAUTHORTEL|(865) 974-9201|
COMMENT     VNTAUTHORWWW|http://botany1.bio.utk.edu|
COMMENT     VNTAUTHORAD1|Univeristy of Tennessee|
COMMENT     VNTAUTHORAD2|Department of Botany|
COMMENT     VNTAUTHORAD3|1406 Circle Dr|
COMMENT     VNTAUTHORAD4|Knoxville, TN 37996-1100|
FEATURES             Location/Qualifiers
     repeat_region   9156..9182
                     /vntifkey="34"
                     /label=LB
                     /note="left border repeat from C58 T-DNA"
     polyA_signal    complement(9266..9517)
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="from mannopine synthase"
     gene            complement(9528..10079)
                     /gene="BAR"
                     /vntifkey="60"
     CDS             complement(9528..10079)
                     /gene="BAR"
                     /codon_start=1
                     /product="phosphinothricin acetyl transferase"
                     /protein_id="AAP78910.1"
                     /db_xref="GI:32265028"
                     /vntifkey="4"
                     /label=BAR-R
                     /note="basta resistance protein"
     promoter        complement(10085..10465)
                     /vntifkey="30"
                     /label=Promoter\(bar)
                     /note="mannopine synthase promoter"
     repeat_region   2878..2901
                     /vntifkey="34"
                     /label=RB
                     /note="right border repeat from C58 T-DNA"
     rep_origin      complement(3963..4963)
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="pVS1"
     rep_origin      complement(7366..7647)
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="pBR325"
     gene            complement(7937..8731)
                     /gene="aadA"
                     /vntifkey="60"
     CDS             complement(7937..8731)
                     /gene="aadA"
                     /function="kanamycin resistance"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAP78911.1"
                     /db_xref="GI:32265029"
                     /vntifkey="4"
                     /label=KAN-R
                     /note="aadA"
     CDS             782..1643
                     /vntifkey="4"
                     /label=PIP2A
     promoter        10..736
                     /vntifkey="29"
                     /label=d35S
                     /note="double 35S promoter"
     terminator      2388..2637
                     /vntifkey="43"
                     /label=nos\3'
                     /note="terminator from nopaline synthase gene"
     primer_bind     672..695
                     /vntifkey="28"
                     /label=35S\primer
     primer_bind     2122..2140
                     /vntifkey="28"
                     /label=SEQ-CFP-F1
                     /note="primer is specific for CFP"
     primer_bind     complement(1841..1858)
                     /vntifkey="28"
                     /label=SEQ-CFP-R1
                     /note="primer is specific for CFP"
     CDS             1649..2368
                     /vntifkey="4"
                     /label=ECFP
                     /note="ECFP coding sequence"
BASE COUNT     2533 a      2881 c      2821 g      2247 t 
ORIGIN
        1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 
       61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 
      121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 
      181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 
      241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 
      301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 
      361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 
      421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 
      481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 
      541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 
      601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 
      661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 
      721 tcatttcatt tggagaggac agcccagatc aactagtctt aagtccggag ctagctctag 
      781 aatggcaaag gatgtggaag ccgttcccgg agaaggattt cagacaagag actatcaaga 
      841 tccgccacca gctccgttta ttgatggagc ggagctaaag aagtggtctt tctacagagc 
      901 agttatcgcc gagttcgtag ccactctcct cttcttatac atcaccgttt tgacagtcat 
      961 cggttacaag attcagtccg atactgatgc cggtggagta gattgcggcg gagttggaat 
     1021 cctcggtatc gcttgggcct ttggtggtat gatcttcatc ctcgtctact gcaccgccgg 
     1081 tatctctggt ggtcacatta acccagcggt gacatttggg ctattcttgg cacgtaaagt 
     1141 gtcgttacct agggccctat tgtacataat cgctcagtgt ttgggtgcga tttgtggagt 
     1201 tggttttgtc aaagccttcc aaagctctta ctacacccgt tacggaggtg gagccaactc 
     1261 tctagccgat ggctacagca cagggaccgg tttagccgca gagatcattg gtactttcgt 
     1321 tcttgtctac actgtcttct ctgctactga ccccaaacgt agtgctagag actcccacgt 
     1381 tccggtgttg gcgccacttc caatcggatt tgccgtgttc atggtacatt tggctaccat 
     1441 tcccattacc ggaaccggaa ttaacccggc aaggagtttc ggagctgccg taatctacaa 
     1501 caagagcaag ccatgggatg accactggat attttgggtt ggaccattca ttggagctgc 
     1561 gatagctgca ttctaccacc aattcgttct gagagcttca ggttctaagt ctctcggatc 
     1621 attcagaagt gctgccaacg tcggatccat ggtgagcaag ggcgaggagc tgttcaccgg 
     1681 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc 
     1741 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac 
     1801 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg 
     1861 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga 
     1921 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc 
     1981 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt 
     2041 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca gccacaacgt 
     2101 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa 
     2161 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga 
     2221 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga 
     2281 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac 
     2341 tctcggcatg gacgagctgt acaagtaaag cggccgcccg gctgcagttc aaacatttgg 
     2401 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt 
     2461 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga 
     2521 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata 
     2581 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa 
     2641 ttcgatatca agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 
     2701 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 
     2761 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgctagag 
     2821 cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga 
     2881 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata acggatattt 
     2941 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt 
     3001 tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc 
     3061 tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga 
     3121 caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag 
     3181 tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc 
     3241 ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa 
     3301 ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac 
     3361 cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc 
     3421 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag 
     3481 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc 
     3541 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc 
     3601 gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc 
     3661 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc 
     3721 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt 
     3781 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac 
     3841 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca 
     3901 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg 
     3961 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc 
     4021 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg 
     4081 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc 
     4141 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg 
     4201 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac 
     4261 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc 
     4321 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg 
     4381 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt 
     4441 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt 
     4501 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga 
     4561 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc 
     4621 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga 
     4681 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc 
     4741 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct 
     4801 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa 
     4861 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg 
     4921 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat 
     4981 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct 
     5041 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt 
     5101 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg 
     5161 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc 
     5221 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg 
     5281 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc 
     5341 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg 
     5401 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta 
     5461 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg 
     5521 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc 
     5581 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag 
     5641 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc 
     5701 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc 
     5761 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag 
     5821 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga 
     5881 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct 
     5941 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt 
     6001 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt 
     6061 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag 
     6121 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag 
     6181 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt 
     6241 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc 
     6301 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg 
     6361 ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga 
     6421 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg 
     6481 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct 
     6541 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg 
     6601 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac 
     6661 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac 
     6721 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc 
     6781 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 
     6841 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 
     6901 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc 
     6961 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
     7021 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct 
     7081 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 
     7141 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 
     7201 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 
     7261 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 
     7321 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 
     7381 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 
     7441 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 
     7501 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 
     7561 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 
     7621 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 
     7681 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 
     7741 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 
     7801 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 
     7861 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 
     7921 tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc 
     7981 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat 
     8041 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc 
     8101 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca 
     8161 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca 
     8221 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca 
     8281 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg 
     8341 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa 
     8401 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac 
     8461 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca 
     8521 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt 
     8581 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata 
     8641 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt 
     8701 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta 
     8761 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc 
     8821 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta 
     8881 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg 
     8941 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc 
     9001 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg 
     9061 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg 
     9121 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac 
     9181 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac 
     9241 gccgaattaa ttcgagctcg gatctgataa tttatttgaa aattcataag aaaagcaaac 
     9301 gttacatgaa ttgatgaaac aatacaaaga cagataaagc cacgcacatt taggatattg 
     9361 gccgagatta ctgaatattg agtaagatca cggaatttct gacaggagca tgtcttcaat 
     9421 tcagcccaaa tggcagttga aatactcaaa ccgccccata tgcaggagcg gatcattcat 
     9481 tgtttgtttg gttgcctttg ccaacatggg agtccaagat tctgcagtta gatctcggtg 
     9541 acgggcagga ccggacgggg cggtaccggc aggctgaagt ccagctgcca gaaacccacg 
     9601 tcatgccagt tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg ggcatatccg 
     9661 agcgcctcgt gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc gaccacgctc 
     9721 ttgaagccct gtgcctccag ggacttcagc aggtgggtgt agagcgtgga gcccagtccc 
     9781 gtccgctggt ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc gtaggcgttg 
     9841 cgtgccttcc aggggcccgc gtaggcgatg ccggcgacct cgccgtccac ctcggcgacg 
     9901 agccagggat agcgctcccg cagacggacg aggtcgtccg tccactcctg cggttcctgc 
     9961 ggctcggtac ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat ggtgcagacc 
    10021 gccggcatgt ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc tgggctcatc 
    10081 gattcgattt ggtgtatcga gattggttat gaaattcaga tgctagtgta atgtattggt 
    10141 aatttgggaa gatataatag gaagcaaggc tatttatcca tttctgaaaa ggcgaaatgg 
    10201 cgtcaccgcg agcgtcacgc gcattccgtt cttgctgtaa agcgttgttt ggtacacttt 
    10261 tgactagcga ggcttggcgt gtcagcgtat ctattcaaaa gtcgttaatg gctgcggatc 
    10321 aagaaaaagt tggaatagaa acagaatacc cgcgaaattc aggcccggtt gccatgtcct 
    10381 acacgccgaa ataaacgacc aaattagtag aaaaataaaa actagctcag atacttacgt 
    10441 cacgtcttgc gcactgattt gaaaaatctc agaattcgag ct 
//
