LOCUS pm-cb 10482 bp DNA circular 18-OCT-2007 DEFINITION pAN623. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|447858999| COMMENT VNTDBDATE|460547385| COMMENT VNTNAME|pm-cb| COMMENT VNTAUTHORNAME|NebenfŸhr Lab| COMMENT VNTAUTHORTEL|(865) 974-9201| COMMENT VNTAUTHORWWW|http://botany1.bio.utk.edu| COMMENT VNTAUTHORAD1|Univeristy of Tennessee| COMMENT VNTAUTHORAD2|Department of Botany| COMMENT VNTAUTHORAD3|1406 Circle Dr| COMMENT VNTAUTHORAD4|Knoxville, TN 37996-1100| FEATURES Location/Qualifiers repeat_region 9156..9182 /vntifkey="34" /label=LB /note="left border repeat from C58 T-DNA" polyA_signal complement(9266..9517) /vntifkey="25" /label=PolyA_Signal_1 /note="from mannopine synthase" gene complement(9528..10079) /gene="BAR" /vntifkey="60" CDS complement(9528..10079) /gene="BAR" /codon_start=1 /product="phosphinothricin acetyl transferase" /protein_id="AAP78910.1" /db_xref="GI:32265028" /vntifkey="4" /label=BAR-R /note="basta resistance protein" promoter complement(10085..10465) /vntifkey="30" /label=Promoter\(bar) /note="mannopine synthase promoter" repeat_region 2878..2901 /vntifkey="34" /label=RB /note="right border repeat from C58 T-DNA" rep_origin complement(3963..4963) /vntifkey="33" /label=Rep_Origin_1 /note="pVS1" rep_origin complement(7366..7647) /vntifkey="33" /label=Rep_Origin_2 /note="pBR325" gene complement(7937..8731) /gene="aadA" /vntifkey="60" CDS complement(7937..8731) /gene="aadA" /function="kanamycin resistance" /codon_start=1 /product="aminoglycoside phosphotransferase" /protein_id="AAP78911.1" /db_xref="GI:32265029" /vntifkey="4" /label=KAN-R /note="aadA" CDS 782..1643 /vntifkey="4" /label=PIP2A promoter 10..736 /vntifkey="29" /label=d35S /note="double 35S promoter" terminator 2388..2637 /vntifkey="43" /label=nos\3' /note="terminator from nopaline synthase gene" primer_bind 672..695 /vntifkey="28" /label=35S\primer primer_bind 2122..2140 /vntifkey="28" /label=SEQ-CFP-F1 /note="primer is specific for CFP" primer_bind complement(1841..1858) /vntifkey="28" /label=SEQ-CFP-R1 /note="primer is specific for CFP" CDS 1649..2368 /vntifkey="4" /label=ECFP /note="ECFP coding sequence" BASE COUNT 2533 a 2881 c 2821 g 2247 t ORIGIN 1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 721 tcatttcatt tggagaggac agcccagatc aactagtctt aagtccggag ctagctctag 781 aatggcaaag gatgtggaag ccgttcccgg agaaggattt cagacaagag actatcaaga 841 tccgccacca gctccgttta ttgatggagc ggagctaaag aagtggtctt tctacagagc 901 agttatcgcc gagttcgtag ccactctcct cttcttatac atcaccgttt tgacagtcat 961 cggttacaag attcagtccg atactgatgc cggtggagta gattgcggcg gagttggaat 1021 cctcggtatc gcttgggcct ttggtggtat gatcttcatc ctcgtctact gcaccgccgg 1081 tatctctggt ggtcacatta acccagcggt gacatttggg ctattcttgg cacgtaaagt 1141 gtcgttacct agggccctat tgtacataat cgctcagtgt ttgggtgcga tttgtggagt 1201 tggttttgtc aaagccttcc aaagctctta ctacacccgt tacggaggtg gagccaactc 1261 tctagccgat ggctacagca cagggaccgg tttagccgca gagatcattg gtactttcgt 1321 tcttgtctac actgtcttct ctgctactga ccccaaacgt agtgctagag actcccacgt 1381 tccggtgttg gcgccacttc caatcggatt tgccgtgttc atggtacatt tggctaccat 1441 tcccattacc ggaaccggaa ttaacccggc aaggagtttc ggagctgccg taatctacaa 1501 caagagcaag ccatgggatg accactggat attttgggtt ggaccattca ttggagctgc 1561 gatagctgca ttctaccacc aattcgttct gagagcttca ggttctaagt ctctcggatc 1621 attcagaagt gctgccaacg tcggatccat ggtgagcaag ggcgaggagc tgttcaccgg 1681 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc 1741 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac 1801 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg 1861 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga 1921 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc 1981 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt 2041 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca gccacaacgt 2101 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa 2161 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga 2221 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga 2281 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac 2341 tctcggcatg gacgagctgt acaagtaaag cggccgcccg gctgcagttc aaacatttgg 2401 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt 2461 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga 2521 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata 2581 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa 2641 ttcgatatca agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 2701 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 2761 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgctagag 2821 cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga 2881 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata acggatattt 2941 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt 3001 tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc 3061 tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga 3121 caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag 3181 tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc 3241 ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa 3301 ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac 3361 cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc 3421 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag 3481 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc 3541 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc 3601 gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc 3661 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc 3721 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt 3781 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac 3841 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca 3901 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg 3961 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc 4021 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg 4081 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc 4141 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg 4201 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac 4261 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc 4321 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg 4381 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt 4441 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt 4501 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga 4561 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc 4621 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga 4681 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc 4741 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct 4801 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa 4861 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg 4921 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat 4981 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct 5041 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt 5101 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg 5161 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc 5221 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg 5281 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc 5341 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg 5401 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta 5461 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg 5521 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc 5581 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag 5641 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc 5701 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc 5761 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag 5821 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga 5881 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct 5941 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt 6001 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt 6061 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag 6121 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag 6181 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt 6241 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc 6301 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg 6361 ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga 6421 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg 6481 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct 6541 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg 6601 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac 6661 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac 6721 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc 6781 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 6841 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 6901 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc 6961 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 7021 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct 7081 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 7141 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 7201 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 7261 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 7321 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 7381 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 7441 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 7501 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 7561 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 7621 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 7681 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 7741 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 7801 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 7861 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 7921 tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc 7981 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat 8041 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc 8101 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca 8161 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca 8221 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca 8281 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg 8341 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa 8401 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac 8461 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca 8521 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt 8581 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata 8641 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt 8701 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta 8761 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc 8821 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta 8881 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg 8941 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc 9001 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg 9061 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg 9121 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac 9181 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac 9241 gccgaattaa ttcgagctcg gatctgataa tttatttgaa aattcataag aaaagcaaac 9301 gttacatgaa ttgatgaaac aatacaaaga cagataaagc cacgcacatt taggatattg 9361 gccgagatta ctgaatattg agtaagatca cggaatttct gacaggagca tgtcttcaat 9421 tcagcccaaa tggcagttga aatactcaaa ccgccccata tgcaggagcg gatcattcat 9481 tgtttgtttg gttgcctttg ccaacatggg agtccaagat tctgcagtta gatctcggtg 9541 acgggcagga ccggacgggg cggtaccggc aggctgaagt ccagctgcca gaaacccacg 9601 tcatgccagt tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg ggcatatccg 9661 agcgcctcgt gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc gaccacgctc 9721 ttgaagccct gtgcctccag ggacttcagc aggtgggtgt agagcgtgga gcccagtccc 9781 gtccgctggt ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc gtaggcgttg 9841 cgtgccttcc aggggcccgc gtaggcgatg ccggcgacct cgccgtccac ctcggcgacg 9901 agccagggat agcgctcccg cagacggacg aggtcgtccg tccactcctg cggttcctgc 9961 ggctcggtac ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat ggtgcagacc 10021 gccggcatgt ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc tgggctcatc 10081 gattcgattt ggtgtatcga gattggttat gaaattcaga tgctagtgta atgtattggt 10141 aatttgggaa gatataatag gaagcaaggc tatttatcca tttctgaaaa ggcgaaatgg 10201 cgtcaccgcg agcgtcacgc gcattccgtt cttgctgtaa agcgttgttt ggtacacttt 10261 tgactagcga ggcttggcgt gtcagcgtat ctattcaaaa gtcgttaatg gctgcggatc 10321 aagaaaaagt tggaatagaa acagaatacc cgcgaaattc aggcccggtt gccatgtcct 10381 acacgccgaa ataaacgacc aaattagtag aaaaataaaa actagctcag atacttacgt 10441 cacgtcttgc gcactgattt gaaaaatctc agaattcgag ct //