LOCUS       mt-yb        9708 bp    DNA   circular            18-OCT-2007
DEFINITION  pAN139.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|446570186|
COMMENT     VNTDBDATE|460547292|
COMMENT     VNTNAME|mt-yb|
COMMENT     VNTAUTHORNAME|NebenfŸhr Lab|
COMMENT     VNTAUTHORTEL|(865) 974-9201|
COMMENT     VNTAUTHORWWW|http://botany1.bio.utk.edu|
COMMENT     VNTAUTHORAD1|Univeristy of Tennessee|
COMMENT     VNTAUTHORAD2|Department of Botany|
COMMENT     VNTAUTHORAD3|1406 Circle Dr|
COMMENT     VNTAUTHORAD4|Knoxville, TN 37996-1100|
FEATURES             Location/Qualifiers
     repeat_region   8382..8408
                     /vntifkey="34"
                     /label=LB
                     /note="left border repeat from C58 T-DNA"
     polyA_signal    complement(8492..8743)
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="from mannopine synthase"
     gene            complement(8754..9305)
                     /gene="BAR"
                     /vntifkey="60"
     CDS             complement(8754..9305)
                     /gene="BAR"
                     /codon_start=1
                     /product="phosphinothricin acetyl transferase"
                     /protein_id="AAP78910.1"
                     /db_xref="GI:32265028"
                     /vntifkey="4"
                     /label=BAR-R
                     /note="basta resistance protein"
     promoter        complement(9311..9691)
                     /vntifkey="30"
                     /label=Promoter\(bar)
                     /note="mannopine synthase promoter"
     repeat_region   2104..2127
                     /vntifkey="34"
                     /label=RB
                     /note="right border repeat from C58 T-DNA"
     rep_origin      complement(3189..4189)
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="pVS1"
     rep_origin      complement(6592..6873)
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="pBR325"
     gene            complement(7163..7957)
                     /gene="aadA"
                     /vntifkey="60"
     CDS             complement(7163..7957)
                     /gene="aadA"
                     /function="kanamycin resistance"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAP78911.1"
                     /db_xref="GI:32265029"
                     /vntifkey="4"
                     /label=KAN-R
                     /note="aadA"
     CDS             782..868
                     /vntifkey="4"
                     /label=COX4ts
                     /note="mitochondrial targeting sequence from ScCOX4"
     primer_bind     complement(1844..1864)
                     /vntifkey="28"
                     /label=EXCA-R1
     primer_bind     673..696
                     /vntifkey="28"
                     /label=35S\primer
     promoter        10..736
                     /vntifkey="29"
                     /label=d35S
                     /note="double 35S promoter"
     terminator      1615..1864
                     /vntifkey="43"
                     /label=nos\3'
                     /note="terminator from nopaline synthase gene"
     CDS             875..1594
                     /vntifkey="4"
                     /label=EYFP
                     /note="EYFP coding sequence"
     primer_bind     complement(928..950)
                     /vntifkey="28"
                     /label=SEQ-GFP-NR
                     /note="annealing site of GFP sequencing primer"
BASE COUNT     2371 a      2685 c      2616 g      2036 t 
ORIGIN
        1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 
       61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 
      121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 
      181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 
      241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 
      301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 
      361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 
      421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 
      481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 
      541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 
      601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 
      661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 
      721 tcatttcatt tggagaggac agcccagatc aactagtctt aagtccggag ctagctctag 
      781 aatgctttca ctacgtcaat ctataagatt tttcaagcca gccacaagaa ctttgtgtag 
      841 ctctagatat ctgcttcagc aaaaacccgg atccatggtg agcaagggcg aggagctgtt 
      901 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag 
      961 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg 
     1021 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct 
     1081 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat 
     1141 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac 
     1201 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat 
     1261 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca 
     1321 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg 
     1381 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat 
     1441 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag 
     1501 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg 
     1561 gatcactctc ggcatggacg agctgtacaa gtaaagcggc cgcccggctg cagttcaaac 
     1621 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata 
     1681 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt 
     1741 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac 
     1801 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat 
     1861 cgggaattcg atatcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa 
     1921 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 
     1981 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 
     2041 ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt 
     2101 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataacgg 
     2161 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca 
     2221 cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta tagtgcagtc 
     2281 ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt 
     2341 acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc 
     2401 ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc 
     2461 gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg 
     2521 gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg 
     2581 cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca 
     2641 gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc 
     2701 atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg 
     2761 ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta 
     2821 atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc 
     2881 ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa 
     2941 ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc 
     3001 gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt 
     3061 gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa 
     3121 caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg 
     3181 aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc 
     3241 ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct 
     3301 tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca 
     3361 gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg 
     3421 ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat 
     3481 cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc 
     3541 cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt 
     3601 tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt 
     3661 cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc 
     3721 cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct 
     3781 ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt 
     3841 cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg 
     3901 gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc 
     3961 cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca 
     4021 ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg 
     4081 agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca 
     4141 acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg 
     4201 gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg 
     4261 ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg 
     4321 aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt 
     4381 ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg 
     4441 ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgac ggtcgcaaac 
     4501 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg 
     4561 ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc 
     4621 aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt 
     4681 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg 
     4741 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc 
     4801 gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt 
     4861 ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt 
     4921 cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg 
     4981 tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc 
     5041 agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc 
     5101 gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta 
     5161 gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag 
     5221 ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc 
     5281 ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg 
     5341 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg 
     5401 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc 
     5461 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc 
     5521 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc 
     5581 aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca 
     5641 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt 
     5701 acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt 
     5761 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac 
     5821 tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct 
     5881 ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg 
     5941 gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc 
     6001 ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga 
     6061 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa 
     6121 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca 
     6181 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga 
     6241 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 
     6301 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 
     6361 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 
     6421 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 
     6481 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 
     6541 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 
     6601 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 
     6661 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 
     6721 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 
     6781 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 
     6841 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 
     6901 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 
     6961 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 
     7021 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 
     7081 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 
     7141 ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa tattttattt 
     7201 tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc 
     7261 cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc 
     7321 cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt 
     7381 ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat 
     7441 catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat 
     7501 cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg 
     7561 tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct 
     7621 cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg 
     7681 cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa 
     7741 caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg 
     7801 tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct 
     7861 tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca 
     7921 gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct 
     7981 acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt 
     8041 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt 
     8101 ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac 
     8161 gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc 
     8221 ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt 
     8281 acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat 
     8341 tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt 
     8401 gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactga 
     8461 attaacgccg aattaattcg agctcggatc tgataattta tttgaaaatt cataagaaaa 
     8521 gcaaacgtta catgaattga tgaaacaata caaagacaga taaagccacg cacatttagg 
     8581 atattggccg agattactga atattgagta agatcacgga atttctgaca ggagcatgtc 
     8641 ttcaattcag cccaaatggc agttgaaata ctcaaaccgc cccatatgca ggagcggatc 
     8701 attcattgtt tgtttggttg cctttgccaa catgggagtc caagattctg cagttagatc 
     8761 tcggtgacgg gcaggaccgg acggggcggt accggcaggc tgaagtccag ctgccagaaa 
     8821 cccacgtcat gccagttccc gtgcttgaag ccggccgccc gcagcatgcc gcggggggca 
     8881 tatccgagcg cctcgtgcat gcgcacgctc gggtcgttgg gcagcccgat gacagcgacc 
     8941 acgctcttga agccctgtgc ctccagggac ttcagcaggt gggtgtagag cgtggagccc 
     9001 agtcccgtcc gctggtggcg gggggagacg tacacggtcg actcggccgt ccagtcgtag 
     9061 gcgttgcgtg ccttccaggg gcccgcgtag gcgatgccgg cgacctcgcc gtccacctcg 
     9121 gcgacgagcc agggatagcg ctcccgcaga cggacgaggt cgtccgtcca ctcctgcggt 
     9181 tcctgcggct cggtacggaa gttgaccgtg cttgtctcga tgtagtggtt gacgatggtg 
     9241 cagaccgccg gcatgtccgc ctcggtggca cggcggatgt cggccgggcg tcgttctggg 
     9301 ctcatcgatt cgatttggtg tatcgagatt ggttatgaaa ttcagatgct agtgtaatgt 
     9361 attggtaatt tgggaagata taataggaag caaggctatt tatccatttc tgaaaaggcg 
     9421 aaatggcgtc accgcgagcg tcacgcgcat tccgttcttg ctgtaaagcg ttgtttggta 
     9481 cacttttgac tagcgaggct tggcgtgtca gcgtatctat tcaaaagtcg ttaatggctg 
     9541 cggatcaaga aaaagttgga atagaaacag aatacccgcg aaattcaggc ccggttgcca 
     9601 tgtcctacac gccgaaataa acgaccaaat tagtagaaaa ataaaaacta gctcagatac 
     9661 ttacgtcacg tcttgcgcac tgatttgaaa aatctcagaa ttcgagct 
//
