LOCUS       mt-rb        9699 bp    DNA   circular            18-OCT-2007
DEFINITION  pAN613.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|443790927|
COMMENT     VNTDBDATE|460547197|
COMMENT     VNTNAME|mt-rb|
COMMENT     VNTAUTHORNAME|NebenfŸhr Lab|
COMMENT     VNTAUTHORTEL|(865) 974-9201|
COMMENT     VNTAUTHORWWW|http://botany1.bio.utk.edu|
COMMENT     VNTAUTHORAD1|Univeristy of Tennessee|
COMMENT     VNTAUTHORAD2|Department of Botany|
COMMENT     VNTAUTHORAD3|1406 Circle Dr|
COMMENT     VNTAUTHORAD4|Knoxville, TN 37996-1100|
FEATURES             Location/Qualifiers
     repeat_region   8373..8399
                     /vntifkey="34"
                     /label=LB
                     /note="left border repeat from C58 T-DNA"
     polyA_signal    complement(8483..8734)
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="from mannopine synthase"
     gene            complement(8745..9296)
                     /gene="BAR"
                     /vntifkey="60"
     CDS             complement(8745..9296)
                     /gene="BAR"
                     /codon_start=1
                     /product="phosphinothricin acetyl transferase"
                     /protein_id="AAP78910.1"
                     /db_xref="GI:32265028"
                     /vntifkey="4"
                     /label=BAR-R
                     /note="basta resistance protein"
     promoter        complement(9302..9682)
                     /vntifkey="30"
                     /label=Promoter\(bar)
                     /note="mannopine synthase promoter"
     repeat_region   2095..2118
                     /vntifkey="34"
                     /label=RB
                     /note="right border repeat from C58 T-DNA"
     rep_origin      complement(3180..4180)
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="pVS1"
     rep_origin      complement(6583..6864)
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="pBR325"
     gene            complement(7154..7948)
                     /gene="aadA"
                     /vntifkey="60"
     CDS             complement(7154..7948)
                     /gene="aadA"
                     /function="kanamycin resistance"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAP78911.1"
                     /db_xref="GI:32265029"
                     /vntifkey="4"
                     /label=KAN-R
                     /note="aadA"
     CDS             782..874
                     /vntifkey="4"
                     /label=Cox4ts
                     /note="mitochondrial targeting sequence from yeast (S. cerevisiae) cytochrome oxidase IV"
     CDS             875..1585
                     /vntifkey="4"
                     /label=mCherry
                     /note="red fluorescent protein"
     primer_bind     673..696
                     /vntifkey="28"
                     /label=35S\primer
     promoter        10..736
                     /vntifkey="29"
                     /label=d35S
                     /note="double 35S promoter"
     terminator      1606..1855
                     /vntifkey="43"
                     /label=nos\3'
                     /note="terminator from nopaline synthase gene"
BASE COUNT     2364 a      2670 c      2633 g      2032 t 
ORIGIN
        1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 
       61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 
      121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 
      181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 
      241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 
      301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 
      361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 
      421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 
      481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 
      541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 
      601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 
      661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 
      721 tcatttcatt tggagaggac agcccagatc aactagtctt aagtccggag ctagctctag 
      781 aatgctttca ctacgtcaat ctataagatt tttcaagcca gccacaagaa ctttgtgtag 
      841 ctctagatat ctgcttcagc aaaaacccgg atccatggtg agcaagggcg aggaggataa 
      901 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 
      961 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 
     1021 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 
     1081 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 
     1141 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 
     1201 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct 
     1261 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga 
     1321 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 
     1381 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa 
     1441 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca 
     1501 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg 
     1561 cggcatggac gagctgtaca agtaaagcgg ccgcccggct gcagttcaaa catttggcaa 
     1621 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg 
     1681 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg 
     1741 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag 
     1801 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattc 
     1861 gatatcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt 
     1921 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga 
     1981 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag 
     2041 cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag 
     2101 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa 
     2161 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc 
     2221 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga 
     2281 cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag 
     2341 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag 
     2401 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg 
     2461 ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg 
     2521 cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc 
     2581 ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg 
     2641 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag 
     2701 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc 
     2761 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac 
     2821 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct 
     2881 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc 
     2941 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag 
     3001 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca 
     3061 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga 
     3121 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga 
     3181 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg 
     3241 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg 
     3301 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc 
     3361 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat 
     3421 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca 
     3481 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca 
     3541 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat 
     3601 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat 
     3661 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt 
     3721 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct 
     3781 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg 
     3841 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct 
     3901 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg 
     3961 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc 
     4021 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc 
     4081 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc 
     4141 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac 
     4201 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa 
     4261 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc 
     4321 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc 
     4381 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg 
     4441 caatggcact ggaaccccca agcccgagga atcggcgtga cggtcgcaaa ccatccggcc 
     4501 cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg 
     4561 ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg 
     4621 ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga 
     4681 agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca 
     4741 cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac 
     4801 gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc 
     4861 cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa 
     4921 ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc 
     4981 cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg 
     5041 acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga 
     5101 aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca 
     5161 agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga 
     5221 tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact 
     5281 ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca 
     5341 aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt 
     5401 tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg 
     5461 atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga 
     5521 tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc 
     5581 tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc 
     5641 caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga 
     5701 accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa 
     5761 actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc 
     5821 agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc 
     5881 ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc 
     5941 caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac 
     6001 atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag 
     6061 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag 
     6121 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat 
     6181 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc 
     6241 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt 
     6301 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 
     6361 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 
     6421 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 
     6481 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 
     6541 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 
     6601 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 
     6661 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 
     6721 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 
     6781 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 
     6841 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 
     6901 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 
     6961 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 
     7021 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 
     7081 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 
     7141 tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat 
     7201 caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct 
     7261 ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc 
     7321 caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt 
     7381 cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct 
     7441 cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat 
     7501 cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac 
     7561 aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct 
     7621 tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca 
     7681 tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct 
     7741 ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat 
     7801 accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct 
     7861 tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca 
     7921 attccggtga tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt 
     7981 aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt 
     8041 ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa 
     8101 catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca 
     8161 tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta 
     8221 gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct 
     8281 ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg 
     8341 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa 
     8401 ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg aattaacgcc 
     8461 gaattaattc gagctcggat ctgataattt atttgaaaat tcataagaaa agcaaacgtt 
     8521 acatgaattg atgaaacaat acaaagacag ataaagccac gcacatttag gatattggcc 
     8581 gagattactg aatattgagt aagatcacgg aatttctgac aggagcatgt cttcaattca 
     8641 gcccaaatgg cagttgaaat actcaaaccg ccccatatgc aggagcggat cattcattgt 
     8701 ttgtttggtt gcctttgcca acatgggagt ccaagattct gcagttagat ctcggtgacg 
     8761 ggcaggaccg gacggggcgg taccggcagg ctgaagtcca gctgccagaa acccacgtca 
     8821 tgccagttcc cgtgcttgaa gccggccgcc cgcagcatgc cgcggggggc atatccgagc 
     8881 gcctcgtgca tgcgcacgct cgggtcgttg ggcagcccga tgacagcgac cacgctcttg 
     8941 aagccctgtg cctccaggga cttcagcagg tgggtgtaga gcgtggagcc cagtcccgtc 
     9001 cgctggtggc ggggggagac gtacacggtc gactcggccg tccagtcgta ggcgttgcgt 
     9061 gccttccagg ggcccgcgta ggcgatgccg gcgacctcgc cgtccacctc ggcgacgagc 
     9121 cagggatagc gctcccgcag acggacgagg tcgtccgtcc actcctgcgg ttcctgcggc 
     9181 tcggtacgga agttgaccgt gcttgtctcg atgtagtggt tgacgatggt gcagaccgcc 
     9241 ggcatgtccg cctcggtggc acggcggatg tcggccgggc gtcgttctgg gctcatcgat 
     9301 tcgatttggt gtatcgagat tggttatgaa attcagatgc tagtgtaatg tattggtaat 
     9361 ttgggaagat ataataggaa gcaaggctat ttatccattt ctgaaaaggc gaaatggcgt 
     9421 caccgcgagc gtcacgcgca ttccgttctt gctgtaaagc gttgtttggt acacttttga 
     9481 ctagcgaggc ttggcgtgtc agcgtatcta ttcaaaagtc gttaatggct gcggatcaag 
     9541 aaaaagttgg aatagaaaca gaatacccgc gaaattcagg cccggttgcc atgtcctaca 
     9601 cgccgaaata aacgaccaaa ttagtagaaa aataaaaact agctcagata cttacgtcac 
     9661 gtcttgcgca ctgatttgaa aaatctcaga attcgagct 
//
