LOCUS       G-yb         9772 bp    DNA   circular            18-OCT-2007
DEFINITION  pAN231.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|446466416|
COMMENT     VNTDBDATE|460546942|
COMMENT     VNTNAME|G-yb|
COMMENT     VNTAUTHORNAME|NebenfŸhr Lab|
COMMENT     VNTAUTHORTEL|(865) 974-9201|
COMMENT     VNTAUTHORWWW|http://botany1.bio.utk.edu|
COMMENT     VNTAUTHORAD1|Univeristy of Tennessee|
COMMENT     VNTAUTHORAD2|Department of Botany|
COMMENT     VNTAUTHORAD3|1406 Circle Dr|
COMMENT     VNTAUTHORAD4|Knoxville, TN 37996-1100|
FEATURES             Location/Qualifiers
     repeat_region   8446..8472
                     /vntifkey="34"
                     /label=LB
                     /note="left border repeat from C58 T-DNA"
     polyA_signal    complement(8556..8807)
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="from mannopine synthase"
     gene            complement(8818..9369)
                     /gene="BAR"
                     /vntifkey="60"
     CDS             complement(8818..9369)
                     /gene="BAR"
                     /codon_start=1
                     /product="phosphinothricin acetyl transferase"
                     /protein_id="AAP78910.1"
                     /db_xref="GI:32265028"
                     /vntifkey="4"
                     /label=BAR-R
                     /note="basta resistance protein"
     promoter        complement(9375..9755)
                     /vntifkey="30"
                     /label=Promoter\(bar)
                     /note="mannopine synthase promoter"
     repeat_region   2168..2191
                     /vntifkey="34"
                     /label=RB
                     /note="right border repeat from C58 T-DNA"
     rep_origin      complement(3253..4253)
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="pVS1"
     rep_origin      complement(6656..6937)
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="pBR325"
     gene            complement(7227..8021)
                     /gene="aadA"
                     /vntifkey="60"
     CDS             complement(7227..8021)
                     /gene="aadA"
                     /function="kanamycin resistance"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAP78911.1"
                     /db_xref="GI:32265029"
                     /vntifkey="4"
                     /label=KAN-R
                     /note="aadA"
     CDS             762..911
                     /vntifkey="4"
                     /label=SM1\(t-TM)
                     /note="cytoplasmic tail + TM domain of soybean mannosidase"
     promoter        10..736
                     /vntifkey="29"
                     /label=d35S
                     /note="double 35S promoter"
     terminator      1679..1928
                     /vntifkey="43"
                     /label=nos\3'
                     /note="terminator from nopaline synthase gene"
     CDS             939..1658
                     /vntifkey="4"
                     /label=EYFP
                     /note="EYFP coding sequence"
     primer_bind     complement(992..1014)
                     /vntifkey="28"
                     /label=SEQ-GFP-NR
                     /note="annealing site of GFP sequencing primer"
BASE COUNT     2369 a      2700 c      2649 g      2054 t 
ORIGIN
        1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 
       61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 
      121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 
      181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 
      241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 
      301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 
      361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 
      421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 
      481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 
      541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 
      601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 
      661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 
      721 tcatttcatt tggagaggac agcccagatc tttgtttggg aatggctagc gggagcagat 
      781 cagtgggtag cagcagcagc aaatggaggt actgcaaccc tactagttac ttgaagcgcc 
      841 caaagcgtct tgctctgctc ttcatcgttt tcgtttgtgt ctctttcgtt ttctgggacc 
      901 gtcaaactga cgtctcgagg accggtcccg ggggatccat ggtgagcaag ggcgaggagc 
      961 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 
     1021 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca 
     1081 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg 
     1141 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 
     1201 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 
     1261 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 
     1321 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca 
     1381 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga 
     1441 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 
     1501 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc 
     1561 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 
     1621 ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcccg gctgcagttc 
     1681 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat 
     1741 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt 
     1801 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga 
     1861 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact 
     1921 agatcgggaa ttcgatatca agcttggcac tggccgtcgt tttacaacgt cgtgactggg 
     1981 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc 
     2041 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg 
     2101 aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaactat 
     2161 cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata 
     2221 acggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca 
     2281 accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc 
     2341 agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta 
     2401 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag 
     2461 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc 
     2521 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca 
     2581 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac 
     2641 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt 
     2701 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga 
     2761 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac 
     2821 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc 
     2881 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt 
     2941 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca 
     3001 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta 
     3061 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt 
     3121 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga 
     3181 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag 
     3241 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc 
     3301 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc 
     3361 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa 
     3421 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc 
     3481 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga 
     3541 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg 
     3601 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa 
     3661 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg 
     3721 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg 
     3781 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg 
     3841 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct 
     3901 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg 
     3961 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca 
     4021 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg 
     4081 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa 
     4141 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc 
     4201 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc 
     4261 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga 
     4321 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta 
     4381 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg 
     4441 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg 
     4501 tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgacggtcgc 
     4561 aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg 
     4621 aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg 
     4681 tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg 
     4741 ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc 
     4801 tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg 
     4861 aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag 
     4921 gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg 
     4981 gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc 
     5041 cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga 
     5101 aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg 
     5161 cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg 
     5221 attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag 
     5281 ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt 
     5341 caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc 
     5401 cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc 
     5461 agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac 
     5521 ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc 
     5581 taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta 
     5641 gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg 
     5701 tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag 
     5761 ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat 
     5821 ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca 
     5881 taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg 
     5941 cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg 
     6001 gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac 
     6061 cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct 
     6121 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 
     6181 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca 
     6241 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta 
     6301 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc 
     6361 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 
     6421 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 
     6481 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 
     6541 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 
     6601 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 
     6661 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 
     6721 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 
     6781 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 
     6841 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 
     6901 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 
     6961 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 
     7021 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 
     7081 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 
     7141 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 
     7201 gggattttgg tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt 
     7261 attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct 
     7321 tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc 
     7381 ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg 
     7441 ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa 
     7501 aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc 
     7561 acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta 
     7621 ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac 
     7681 agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca 
     7741 tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt 
     7801 ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag 
     7861 gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc 
     7921 agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg 
     7981 atcagttttt tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt 
     8041 ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac 
     8101 tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa 
     8161 agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag 
     8221 caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa 
     8281 acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg 
     8341 ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg 
     8401 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg 
     8461 tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta 
     8521 ctgaattaac gccgaattaa ttcgagctcg gatctgataa tttatttgaa aattcataag 
     8581 aaaagcaaac gttacatgaa ttgatgaaac aatacaaaga cagataaagc cacgcacatt 
     8641 taggatattg gccgagatta ctgaatattg agtaagatca cggaatttct gacaggagca 
     8701 tgtcttcaat tcagcccaaa tggcagttga aatactcaaa ccgccccata tgcaggagcg 
     8761 gatcattcat tgtttgtttg gttgcctttg ccaacatggg agtccaagat tctgcagtta 
     8821 gatctcggtg acgggcagga ccggacgggg cggtaccggc aggctgaagt ccagctgcca 
     8881 gaaacccacg tcatgccagt tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg 
     8941 ggcatatccg agcgcctcgt gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc 
     9001 gaccacgctc ttgaagccct gtgcctccag ggacttcagc aggtgggtgt agagcgtgga 
     9061 gcccagtccc gtccgctggt ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc 
     9121 gtaggcgttg cgtgccttcc aggggcccgc gtaggcgatg ccggcgacct cgccgtccac 
     9181 ctcggcgacg agccagggat agcgctcccg cagacggacg aggtcgtccg tccactcctg 
     9241 cggttcctgc ggctcggtac ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat 
     9301 ggtgcagacc gccggcatgt ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc 
     9361 tgggctcatc gattcgattt ggtgtatcga gattggttat gaaattcaga tgctagtgta 
     9421 atgtattggt aatttgggaa gatataatag gaagcaaggc tatttatcca tttctgaaaa 
     9481 ggcgaaatgg cgtcaccgcg agcgtcacgc gcattccgtt cttgctgtaa agcgttgttt 
     9541 ggtacacttt tgactagcga ggcttggcgt gtcagcgtat ctattcaaaa gtcgttaatg 
     9601 gctgcggatc aagaaaaagt tggaatagaa acagaatacc cgcgaaattc aggcccggtt 
     9661 gccatgtcct acacgccgaa ataaacgacc aaattagtag aaaaataaaa actagctcag 
     9721 atacttacgt cacgtcttgc gcactgattt gaaaaatctc agaattcgag ct 
//
