LOCUS       G-rb         9763 bp    DNA   circular            18-OCT-2007
DEFINITION  pAN527.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|424124845|
COMMENT     VNTDBDATE|460546836|
COMMENT     VNTNAME|G-rb|
COMMENT     VNTAUTHORNAME|NebenfŸhr Lab|
COMMENT     VNTAUTHORTEL|(865) 974-9201|
COMMENT     VNTAUTHORWWW|http://botany1.bio.utk.edu|
COMMENT     VNTAUTHORAD1|Univeristy of Tennessee|
COMMENT     VNTAUTHORAD2|Department of Botany|
COMMENT     VNTAUTHORAD3|1406 Circle Dr|
COMMENT     VNTAUTHORAD4|Knoxville, TN 37996-1100|
FEATURES             Location/Qualifiers
     CDS             939..1649
                     /vntifkey="4"
                     /label=mCherry
     repeat_region   8437..8463
                     /vntifkey="34"
                     /label=LB
                     /note="left border repeat from C58 T-DNA"
     polyA_signal    complement(8547..8798)
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="from mannopine synthase"
     gene            complement(8809..9360)
                     /gene="BAR"
                     /vntifkey="60"
     CDS             complement(8809..9360)
                     /gene="BAR"
                     /codon_start=1
                     /product="phosphinothricin acetyl transferase"
                     /protein_id="AAP78910.1"
                     /db_xref="GI:32265028"
                     /vntifkey="4"
                     /label=BAR-R
                     /note="basta resistance protein"
     promoter        complement(9366..9746)
                     /vntifkey="30"
                     /label=Promoter\(bar)
                     /note="mannopine synthase promoter"
     repeat_region   2159..2182
                     /vntifkey="34"
                     /label=RB
                     /note="right border repeat from C58 T-DNA"
     rep_origin      complement(3244..4244)
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="pVS1"
     rep_origin      complement(6647..6928)
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="pBR325"
     gene            complement(7218..8012)
                     /gene="aadA"
                     /vntifkey="60"
     CDS             complement(7218..8012)
                     /gene="aadA"
                     /function="kanamycin resistance"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAP78911.1"
                     /db_xref="GI:32265029"
                     /vntifkey="4"
                     /label=KAN-R
                     /note="aadA"
     CDS             762..911
                     /vntifkey="4"
                     /label=SM1\(t-TM)
                     /note="cytoplasmic tail + TM domain of soybean mannosidase"
     promoter        10..736
                     /vntifkey="29"
                     /label=d35S
                     /note="double 35S promoter"
     terminator      1670..1919
                     /vntifkey="43"
                     /label=nos\3'
                     /note="terminator from nopaline synthase gene"
BASE COUNT     2362 a      2685 c      2666 g      2050 t 
ORIGIN
        1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 
       61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 
      121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 
      181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 
      241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 
      301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 
      361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 
      421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 
      481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 
      541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 
      601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 
      661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 
      721 tcatttcatt tggagaggac agcccagatc tttgtttggg aatggctagc gggagcagat 
      781 cagtgggtag cagcagcagc aaatggaggt actgcaaccc tactagttac ttgaagcgcc 
      841 caaagcgtct tgctctgctc ttcatcgttt tcgtttgtgt ctctttcgtt ttctgggacc 
      901 gtcaaactga cgtctcgagg accggtcccg ggggatccat ggtgagcaag ggcgaggagg 
      961 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga 
     1021 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga 
     1081 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc 
     1141 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact 
     1201 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg 
     1261 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga 
     1321 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct 
     1381 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc 
     1441 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg 
     1501 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct 
     1561 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca 
     1621 ccggcggcat ggacgagctg tacaagtaaa gcggccgccc ggctgcagtt caaacatttg 
     1681 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt 
     1741 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag 
     1801 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat 
     1861 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga 
     1921 attcgatatc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg 
     1981 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 
     2041 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga 
     2101 gcagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg 
     2161 acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt 
     2221 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg 
     2281 ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt 
     2341 ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg 
     2401 acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa 
     2461 gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg 
     2521 cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga 
     2581 actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga 
     2641 ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac 
     2701 caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca 
     2761 ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc 
     2821 cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat 
     2881 cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg 
     2941 ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg 
     3001 caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact 
     3061 tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga 
     3121 cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc 
     3181 atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg 
     3241 gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg 
     3301 catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc 
     3361 gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg 
     3421 cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac 
     3481 gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa 
     3541 cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc 
     3601 cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg 
     3661 gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag 
     3721 tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact 
     3781 tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg 
     3841 agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt 
     3901 cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg 
     3961 agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg 
     4021 ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc 
     4081 tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa 
     4141 aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt 
     4201 ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga 
     4261 tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc 
     4321 tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt 
     4381 tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat 
     4441 gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc 
     4501 cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgacggtcg caaaccatcc 
     4561 ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg 
     4621 caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg 
     4681 gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt 
     4741 aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg 
     4801 ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac 
     4861 cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca 
     4921 gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat 
     4981 ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc 
     5041 cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa 
     5101 gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg 
     5161 aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc 
     5221 tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat 
     5281 tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat 
     5341 tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca 
     5401 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga 
     5461 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag 
     5521 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac 
     5581 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt 
     5641 gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg 
     5701 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt 
     5761 gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc 
     5821 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct 
     5881 ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta 
     5941 cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta 
     6001 cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc 
     6061 ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 
     6121 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 
     6181 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag 
     6241 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg 
     6301 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc 
     6361 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 
     6421 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 
     6481 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 
     6541 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 
     6601 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 
     6661 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 
     6721 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 
     6781 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 
     6841 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 
     6901 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 
     6961 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 
     7021 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 
     7081 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 
     7141 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 
     7201 gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc 
     7261 caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata 
     7321 tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt 
     7381 ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc 
     7441 aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac 
     7501 agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc 
     7561 agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag 
     7621 ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata 
     7681 atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca 
     7741 ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc 
     7801 agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct 
     7861 ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat 
     7921 accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt 
     7981 ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct tttctacagt 
     8041 atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg 
     8101 cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt 
     8161 ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct 
     8221 gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag 
     8281 cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac 
     8341 ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt 
     8401 gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa 
     8461 caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa 
     8521 cgccgaatta attcgagctc ggatctgata atttatttga aaattcataa gaaaagcaaa 
     8581 cgttacatga attgatgaaa caatacaaag acagataaag ccacgcacat ttaggatatt 
     8641 ggccgagatt actgaatatt gagtaagatc acggaatttc tgacaggagc atgtcttcaa 
     8701 ttcagcccaa atggcagttg aaatactcaa accgccccat atgcaggagc ggatcattca 
     8761 ttgtttgttt ggttgccttt gccaacatgg gagtccaaga ttctgcagtt agatctcggt 
     8821 gacgggcagg accggacggg gcggtaccgg caggctgaag tccagctgcc agaaacccac 
     8881 gtcatgccag ttcccgtgct tgaagccggc cgcccgcagc atgccgcggg gggcatatcc 
     8941 gagcgcctcg tgcatgcgca cgctcgggtc gttgggcagc ccgatgacag cgaccacgct 
     9001 cttgaagccc tgtgcctcca gggacttcag caggtgggtg tagagcgtgg agcccagtcc 
     9061 cgtccgctgg tggcgggggg agacgtacac ggtcgactcg gccgtccagt cgtaggcgtt 
     9121 gcgtgccttc caggggcccg cgtaggcgat gccggcgacc tcgccgtcca cctcggcgac 
     9181 gagccaggga tagcgctccc gcagacggac gaggtcgtcc gtccactcct gcggttcctg 
     9241 cggctcggta cggaagttga ccgtgcttgt ctcgatgtag tggttgacga tggtgcagac 
     9301 cgccggcatg tccgcctcgg tggcacggcg gatgtcggcc gggcgtcgtt ctgggctcat 
     9361 cgattcgatt tggtgtatcg agattggtta tgaaattcag atgctagtgt aatgtattgg 
     9421 taatttggga agatataata ggaagcaagg ctatttatcc atttctgaaa aggcgaaatg 
     9481 gcgtcaccgc gagcgtcacg cgcattccgt tcttgctgta aagcgttgtt tggtacactt 
     9541 ttgactagcg aggcttggcg tgtcagcgta tctattcaaa agtcgttaat ggctgcggat 
     9601 caagaaaaag ttggaataga aacagaatac ccgcgaaatt caggcccggt tgccatgtcc 
     9661 tacacgccga aataaacgac caaattagta gaaaaataaa aactagctca gatacttacg 
     9721 tcacgtcttg cgcactgatt tgaaaaatct cagaattcga gct 
//
