LOCUS       G-gb         9916 bp    DNA   circular            18-OCT-2007
DEFINITION  pAN232.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|446466209|
COMMENT     VNTDBDATE|460546749|
COMMENT     VNTNAME|G-gb|
COMMENT     VNTAUTHORNAME|NebenfŸhr Lab|
COMMENT     VNTAUTHORTEL|(865) 974-9201|
COMMENT     VNTAUTHORWWW|http://botany1.bio.utk.edu|
COMMENT     VNTAUTHORAD1|Univeristy of Tennessee|
COMMENT     VNTAUTHORAD2|Department of Botany|
COMMENT     VNTAUTHORAD3|1406 Circle Dr|
COMMENT     VNTAUTHORAD4|Knoxville, TN 37996-1100|
FEATURES             Location/Qualifiers
     repeat_region   8590..8616
                     /vntifkey="34"
                     /label=LB
                     /note="left border repeat from C58 T-DNA"
     polyA_signal    complement(8700..8951)
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="from mannopine synthase"
     gene            complement(8962..9513)
                     /gene="BAR"
                     /vntifkey="60"
     CDS             complement(8962..9513)
                     /gene="BAR"
                     /codon_start=1
                     /product="phosphinothricin acetyl transferase"
                     /protein_id="AAP78910.1"
                     /db_xref="GI:32265028"
                     /vntifkey="4"
                     /label=BAR-R
                     /note="basta resistance protein"
     promoter        complement(9519..9899)
                     /vntifkey="30"
                     /label=Promoter\(bar)
                     /note="mannopine synthase promoter"
     repeat_region   2312..2335
                     /vntifkey="34"
                     /label=RB
                     /note="right border repeat from C58 T-DNA"
     rep_origin      complement(3397..4397)
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="pVS1"
     rep_origin      complement(6800..7081)
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="pBR325"
     gene            complement(7371..8165)
                     /gene="aadA"
                     /vntifkey="60"
     CDS             complement(7371..8165)
                     /gene="aadA"
                     /function="kanamycin resistance"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAP78911.1"
                     /db_xref="GI:32265029"
                     /vntifkey="4"
                     /label=KAN-R
                     /note="aadA"
     CDS             762..1056
                     /vntifkey="4"
                     /label=SM1\(t-TM-s)
                     /note="cytoplasmic tail + TM domain + lumenal stalk  from soybean mannosidase"
     promoter        10..736
                     /vntifkey="29"
                     /label=d35S
                     /note="double 35S promoter"
     terminator      1823..2072
                     /vntifkey="43"
                     /label=nos\3'
                     /note="terminator from nopaline synthase gene"
     misc_feature    762..848
                     /vntifkey="21"
                     /label=cytoplasmic\tail
     misc_feature    849..896
                     /vntifkey="21"
                     /label=TransMembrane\domain
     CDS             1083..1802
                     /vntifkey="4"
                     /label=GFP
                     /note="GFP coding region"
BASE COUNT     2422 a      2722 c      2687 g      2085 t 
ORIGIN
        1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 
       61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 
      121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 
      181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 
      241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 
      301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 
      361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 
      421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 
      481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 
      541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 
      601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 
      661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 
      721 tcatttcatt tggagaggac agcccagatc tttgtttggg aatggcgaga gggagcagat 
      781 cagtgggtag cagcagcagc aaatggaggt actgcaaccc ttcctattac ttgaagcgcc 
      841 caaagcgtct tgctctgctc ttcatcgttt tcgtttgtgt ctctttcgtt ttctgggacc 
      901 gtcaaactct cgtcagagag caccaggttg aaatttctga gctgcagaaa gaagtgactg 
      961 atttgaaaaa tttggtggat gatttaaata acaaacaagg tggtacctct gggaagactg 
     1021 acttggggag aaaagctacc aagtccagta aagacgtctc gaggaccggt cccgggggat 
     1081 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 
     1141 acggcgacgt gaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 
     1201 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 
     1261 ccctcgtgac caccttcacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga 
     1321 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 
     1381 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 
     1441 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 
     1501 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga 
     1561 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 
     1621 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 
     1681 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 
     1741 tcctgctgga gttcgtgacc gccgccggga tcactcacgg catggacgag ctgtacaagt 
     1801 aaagcggccg cccggctgca gttcaaacat ttggcaataa agtttcttaa gattgaatcc 
     1861 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat 
     1921 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca 
     1981 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc 
     2041 gcgcgcggtg tcatctatgt tactagatcg ggaattcgat atcaagcttg gcactggccg 
     2101 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 
     2161 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 
     2221 aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg 
     2281 tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa 
     2341 gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt 
     2401 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat 
     2461 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga 
     2521 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg 
     2581 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag 
     2641 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac 
     2701 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct 
     2761 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga 
     2821 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac 
     2881 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct 
     2941 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc 
     3001 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc 
     3061 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc 
     3121 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct 
     3181 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac 
     3241 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc 
     3301 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac 
     3361 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc 
     3421 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat 
     3481 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta 
     3541 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg 
     3601 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc 
     3661 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg 
     3721 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg 
     3781 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg 
     3841 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg 
     3901 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc 
     3961 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca 
     4021 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg 
     4081 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca 
     4141 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac 
     4201 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca 
     4261 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg 
     4321 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat 
     4381 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt 
     4441 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta 
     4501 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa 
     4561 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt 
     4621 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc 
     4681 ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg 
     4741 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag 
     4801 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc 
     4861 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa 
     4921 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg 
     4981 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac 
     5041 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg 
     5101 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg 
     5161 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag 
     5221 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg 
     5281 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg 
     5341 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg 
     5401 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc 
     5461 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc 
     5521 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc 
     5581 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg 
     5641 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag 
     5701 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt 
     5761 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa 
     5821 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg 
     5881 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact 
     5941 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact 
     6001 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa 
     6061 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc 
     6121 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga 
     6181 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg 
     6241 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 
     6301 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 
     6361 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac 
     6421 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 
     6481 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 
     6541 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 
     6601 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 
     6661 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 
     6721 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 
     6781 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 
     6841 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 
     6901 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 
     6961 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 
     7021 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 
     7081 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 
     7141 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 
     7201 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 
     7261 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 
     7321 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat 
     7381 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca 
     7441 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag 
     7501 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact 
     7561 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc 
     7621 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg 
     7681 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat 
     7741 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga 
     7801 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca 
     7861 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca 
     7921 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg 
     7981 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa 
     8041 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta 
     8101 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta 
     8161 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt 
     8221 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa 
     8281 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt 
     8341 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc 
     8401 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat 
     8461 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac 
     8521 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg 
     8581 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa 
     8641 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcgag ctcggatctg 
     8701 ataatttatt tgaaaattca taagaaaagc aaacgttaca tgaattgatg aaacaataca 
     8761 aagacagata aagccacgca catttaggat attggccgag attactgaat attgagtaag 
     8821 atcacggaat ttctgacagg agcatgtctt caattcagcc caaatggcag ttgaaatact 
     8881 caaaccgccc catatgcagg agcggatcat tcattgtttg tttggttgcc tttgccaaca 
     8941 tgggagtcca agattctgca gttagatctc ggtgacgggc aggaccggac ggggcggtac 
     9001 cggcaggctg aagtccagct gccagaaacc cacgtcatgc cagttcccgt gcttgaagcc 
     9061 ggccgcccgc agcatgccgc ggggggcata tccgagcgcc tcgtgcatgc gcacgctcgg 
     9121 gtcgttgggc agcccgatga cagcgaccac gctcttgaag ccctgtgcct ccagggactt 
     9181 cagcaggtgg gtgtagagcg tggagcccag tcccgtccgc tggtggcggg gggagacgta 
     9241 cacggtcgac tcggccgtcc agtcgtaggc gttgcgtgcc ttccaggggc ccgcgtaggc 
     9301 gatgccggcg acctcgccgt ccacctcggc gacgagccag ggatagcgct cccgcagacg 
     9361 gacgaggtcg tccgtccact cctgcggttc ctgcggctcg gtacggaagt tgaccgtgct 
     9421 tgtctcgatg tagtggttga cgatggtgca gaccgccggc atgtccgcct cggtggcacg 
     9481 gcggatgtcg gccgggcgtc gttctgggct catcgattcg atttggtgta tcgagattgg 
     9541 ttatgaaatt cagatgctag tgtaatgtat tggtaatttg ggaagatata ataggaagca 
     9601 aggctattta tccatttctg aaaaggcgaa atggcgtcac cgcgagcgtc acgcgcattc 
     9661 cgttcttgct gtaaagcgtt gtttggtaca cttttgacta gcgaggcttg gcgtgtcagc 
     9721 gtatctattc aaaagtcgtt aatggctgcg gatcaagaaa aagttggaat agaaacagaa 
     9781 tacccgcgaa attcaggccc ggttgccatg tcctacacgc cgaaataaac gaccaaatta 
     9841 gtagaaaaat aaaaactagc tcagatactt acgtcacgtc ttgcgcactg atttgaaaaa 
     9901 tctcagaatt cgagct 
//
