LOCUS       ER-yb        9719 bp    DNA   circular            28-NOV-2007
DEFINITION  pAN118.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|446466031|
COMMENT     VNTDBDATE|464185025|
COMMENT     VNTNAME|ER-yb|
COMMENT     VNTAUTHORNAME|NebenfŸhr Lab|
COMMENT     VNTAUTHORTEL|(865) 974-9201|
COMMENT     VNTAUTHORWWW|http://botany1.bio.utk.edu|
COMMENT     VNTAUTHORAD1|Univeristy of Tennessee|
COMMENT     VNTAUTHORAD2|Department of Botany|
COMMENT     VNTAUTHORAD3|1406 Circle Dr|
COMMENT     VNTAUTHORAD4|Knoxville, TN 37996-1100|
FEATURES             Location/Qualifiers
     repeat_region   8393..8419
                     /vntifkey="34"
                     /label=LB
                     /note="left border repeat from C58 T-DNA"
     polyA_signal    complement(8503..8754)
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="from mannopine synthase"
     gene            complement(8765..9316)
                     /gene="BAR"
                     /vntifkey="60"
     CDS             complement(8765..9316)
                     /gene="BAR"
                     /codon_start=1
                     /product="phosphinothricin acetyl transferase"
                     /protein_id="AAP78910.1"
                     /db_xref="GI:32265028"
                     /vntifkey="4"
                     /label=BAR-R
                     /note="basta resistance protein"
     promoter        complement(9322..9702)
                     /vntifkey="30"
                     /label=Promoter\(bar)
                     /note="mannopine synthase promoter"
     repeat_region   2115..2138
                     /vntifkey="34"
                     /label=RB
                     /note="right border repeat from C58 T-DNA"
     rep_origin      complement(3200..4200)
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="pVS1"
     rep_origin      complement(6603..6884)
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="pBR325"
     gene            complement(7174..7968)
                     /gene="aadA"
                     /vntifkey="60"
     CDS             complement(7174..7968)
                     /gene="aadA"
                     /function="kanamycin resistance"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAP78911.1"
                     /db_xref="GI:32265029"
                     /vntifkey="4"
                     /label=KAN-R
                     /note="aadA"
     promoter        10..736
                     /vntifkey="29"
                     /label=d35S
                     /note="double 35S promoter"
     terminator      1625..1874
                     /vntifkey="43"
                     /label=nos\3'
                     /note="terminator from nopaline synthase gene"
     primer_bind     672..695
                     /vntifkey="28"
                     /label=35S\primer
     sig_peptide     788..856
                     /vntifkey="94"
                     /label=signal\peptide
     CDS             788..1624
                     /vntifkey="4"
                     /label=ER-YFP
                     /note="WAk2 signal pipetid+YFP+HDEL"
BASE COUNT     2369 a      2678 c      2634 g      2038 t 
ORIGIN
        1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 
       61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 
      121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 
      181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 
      241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 
      301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 
      361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 
      421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 
      481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 
      541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 
      601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 
      661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 
      721 tcatttcatt tggagaggac agcccagatc aactagtctt aagtccggag ctagctctag 
      781 agagaagatg aaggtacagg agggtttgtt cgtggtggct gttttctacc ttgcttatac 
      841 gcagctagtc aaggggcaac ctcgcaagga gtgcggatcc atggtgagca agggcgagga 
      901 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 
      961 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 
     1021 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta 
     1081 cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc 
     1141 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 
     1201 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 
     1261 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa 
     1321 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa 
     1381 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 
     1441 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc 
     1501 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 
     1561 cgccgggatc actctcggca tggacgagct gtacaagaga tctcatgacg agctgtaact 
     1621 gcagttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga 
     1681 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca 
     1741 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg 
     1801 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta 
     1861 tgttactaga tcgggaattc gatatcaagc ttggcactgg ccgtcgtttt acaacgtcgt 
     1921 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc 
     1981 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg 
     2041 aatggcgaat gctagagcag cttgagcttg gatcagattg tcgtttcccg ccttcagttt 
     2101 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat 
     2161 tagaataacg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg 
     2221 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct 
     2281 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca 
     2341 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt 
     2401 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca 
     2461 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga 
     2521 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca 
     2581 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg 
     2641 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca 
     2701 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg 
     2761 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg 
     2821 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg 
     2881 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga 
     2941 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga 
     3001 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg 
     3061 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac 
     3121 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac 
     3181 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt 
     3241 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg 
     3301 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt 
     3361 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca 
     3421 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc 
     3481 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg 
     3541 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa 
     3601 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc 
     3661 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg 
     3721 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc 
     3781 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa 
     3841 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag 
     3901 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac 
     3961 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc 
     4021 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta 
     4081 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca 
     4141 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc 
     4201 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca 
     4261 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa 
     4321 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc 
     4381 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc 
     4441 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga 
     4501 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga 
     4561 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg 
     4621 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc 
     4681 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc 
     4741 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg 
     4801 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca 
     4861 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact 
     4921 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa 
     4981 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga 
     5041 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt 
     5101 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga 
     5161 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga 
     5221 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct 
     5281 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct 
     5341 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg 
     5401 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc 
     5461 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt 
     5521 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca 
     5581 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga 
     5641 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa 
     5701 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa 
     5761 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc 
     5821 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg 
     5881 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc 
     5941 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc 
     6001 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg 
     6061 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 
     6121 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca 
     6181 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca 
     6241 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 
     6301 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 
     6361 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 
     6421 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 
     6481 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 
     6541 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 
     6601 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 
     6661 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 
     6721 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 
     6781 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 
     6841 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 
     6901 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 
     6961 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 
     7021 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 
     7081 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 
     7141 acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata 
     7201 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata 
     7261 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt 
     7321 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa 
     7381 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt 
     7441 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc 
     7501 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc 
     7561 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc 
     7621 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag 
     7681 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag 
     7741 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac 
     7801 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc 
     7861 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta 
     7921 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct 
     7981 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc 
     8041 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg 
     8101 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca 
     8161 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt 
     8221 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag 
     8281 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat 
     8341 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga 
     8401 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt 
     8461 taatgtactg aattaacgcc gaattaattc gagctcggat ctgataattt atttgaaaat 
     8521 tcataagaaa agcaaacgtt acatgaattg atgaaacaat acaaagacag ataaagccac 
     8581 gcacatttag gatattggcc gagattactg aatattgagt aagatcacgg aatttctgac 
     8641 aggagcatgt cttcaattca gcccaaatgg cagttgaaat actcaaaccg ccccatatgc 
     8701 aggagcggat cattcattgt ttgtttggtt gcctttgcca acatgggagt ccaagattct 
     8761 gcagttagat ctcggtgacg ggcaggaccg gacggggcgg taccggcagg ctgaagtcca 
     8821 gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc cgcagcatgc 
     8881 cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg ggcagcccga 
     8941 tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg tgggtgtaga 
     9001 gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc gactcggccg 
     9061 tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg gcgacctcgc 
     9121 cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg tcgtccgtcc 
     9181 actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg atgtagtggt 
     9241 tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg tcggccgggc 
     9301 gtcgttctgg gctcatcgat tcgatttggt gtatcgagat tggttatgaa attcagatgc 
     9361 tagtgtaatg tattggtaat ttgggaagat ataataggaa gcaaggctat ttatccattt 
     9421 ctgaaaaggc gaaatggcgt caccgcgagc gtcacgcgca ttccgttctt gctgtaaagc 
     9481 gttgtttggt acacttttga ctagcgaggc ttggcgtgtc agcgtatcta ttcaaaagtc 
     9541 gttaatggct gcggatcaag aaaaagttgg aatagaaaca gaatacccgc gaaattcagg 
     9601 cccggttgcc atgtcctaca cgccgaaata aacgaccaaa ttagtagaaa aataaaaact 
     9661 agctcagata cttacgtcac gtcttgcgca ctgatttgaa aaatctcaga attcgagct 
//
