LOCUS       ER-gb        9710 bp    DNA   circular            11-OCT-2007
DEFINITION  pAN142.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|446465895|
COMMENT     VNTDBDATE|459947555|
COMMENT     VNTNAME|ER-gb|
COMMENT     VNTAUTHORNAME|NebenfŸhr Lab|
COMMENT     VNTAUTHORTEL|(865) 974-9201|
COMMENT     VNTAUTHORWWW|http://botany1.bio.utk.edu|
COMMENT     VNTAUTHORAD1|Univeristy of Tennessee|
COMMENT     VNTAUTHORAD2|Department of Botany|
COMMENT     VNTAUTHORAD3|1406 Circle Dr|
COMMENT     VNTAUTHORAD4|Knoxville, TN 37996-1100|
FEATURES             Location/Qualifiers
     repeat_region   8384..8410
                     /vntifkey="34"
                     /label=LB
                     /note="left border repeat from C58 T-DNA"
     polyA_signal    complement(8494..8745)
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="from mannopine synthase"
     gene            complement(8756..9307)
                     /gene="BAR"
                     /vntifkey="60"
     CDS             complement(8756..9307)
                     /gene="BAR"
                     /codon_start=1
                     /product="phosphinothricin acetyl transferase"
                     /protein_id="AAP78910.1"
                     /db_xref="GI:32265028"
                     /vntifkey="4"
                     /label=BAR-R
                     /note="basta resistance protein"
     promoter        complement(9313..9693)
                     /vntifkey="30"
                     /label=Promoter\(bar)
                     /note="mannopine synthase promoter"
     repeat_region   2106..2129
                     /vntifkey="34"
                     /label=RB
                     /note="right border repeat from C58 T-DNA"
     rep_origin      complement(3191..4191)
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="pVS1"
     rep_origin      complement(6594..6875)
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="pBR325"
     gene            complement(7165..7959)
                     /gene="aadA"
                     /vntifkey="60"
     CDS             complement(7165..7959)
                     /gene="aadA"
                     /function="kanamycin resistance"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAP78911.1"
                     /db_xref="GI:32265029"
                     /vntifkey="4"
                     /label=KAN-R
                     /note="aadA"
     promoter        10..736
                     /vntifkey="29"
                     /label=d35S
                     /note="double 35S promoter"
     terminator      1616..1865
                     /vntifkey="43"
                     /label=nos\3'
                     /note="terminator from nopaline synthase gene"
     primer_bind     672..695
                     /vntifkey="28"
                     /label=35S\primer
     sig_peptide     788..856
                     /vntifkey="94"
                     /label=signal\peptide
     CDS             788..1609
                     /vntifkey="4"
                     /label=ER-GFP5
                     /note="WAK2 signal peptide + GFP5 + HDEL"
BASE COUNT     2414 a      2609 c      2587 g      2100 t 
ORIGIN
        1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 
       61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 
      121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 
      181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 
      241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 
      301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 
      361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 
      421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 
      481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 
      541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 
      601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 
      661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 
      721 tcatttcatt tggagaggac agcccagatc aactagtctt aagtccggag ctagctctag 
      781 agagaagatg aaggtacagg agggtttgtt cgtggtggct gttttctacc ttgcttatac 
      841 gcagctagtc aaggggcaac ctcgcaagga gtgcggatcc atgggtaaag gagaactttt 
      901 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc 
      961 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg 
     1021 cactactgga aaactacctg ttccctggcc aacacttgtc actactttct cttatggtgt 
     1081 tcaatgcttt tcaagatacc cagatcatat gaagcggcac gacttcttca agagcgccat 
     1141 gcctgaggga tacgtgcagg agaggaccat cttcttcaag gacgacggga actacaagac 
     1201 acgtgctgaa gtcaagtttg agggagacac cctcgtcaac aggatcgagc ttaagggaat 
     1261 cgatttcaag gaggacggaa acatcctcgg ccacaagttg gaatacaact acaactccca 
     1321 caacgtatac atcatggccg acaagcaaaa gaacggcatc aaagccaact tcaagacccg 
     1381 ccacaacatc gaagacggcg gcgtgcaact cgctgatcat tatcaacaaa atactccaat 
     1441 tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc 
     1501 gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg 
     1561 gattacacat ggcatggatg aactatacag atctcatgac gagctgtaac tgcagttcaa 
     1621 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca 
     1681 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat 
     1741 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa 
     1801 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag 
     1861 atcgggaatt cgatatcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa 
     1921 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 
     1981 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 
     2041 tgctagagca gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca 
     2101 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataac 
     2161 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac 
     2221 cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag 
     2281 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag 
     2341 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc 
     2401 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg 
     2461 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac 
     2521 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca 
     2581 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga 
     2641 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc 
     2701 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca 
     2761 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc 
     2821 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg 
     2881 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg 
     2941 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc 
     3001 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc 
     3061 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg 
     3121 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat 
     3181 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt 
     3241 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag 
     3301 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa 
     3361 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa 
     3421 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc 
     3481 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat 
     3541 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc 
     3601 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac 
     3661 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca 
     3721 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac 
     3781 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt 
     3841 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc 
     3901 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact 
     3961 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc 
     4021 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa 
     4081 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg 
     4141 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg 
     4201 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc 
     4261 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga 
     4321 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc 
     4381 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc 
     4441 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg acggtcgcaa 
     4501 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa 
     4561 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg 
     4621 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc 
     4681 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta 
     4741 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa 
     4801 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt 
     4861 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt 
     4921 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg 
     4981 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa 
     5041 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca 
     5101 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat 
     5161 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct 
     5221 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca 
     5281 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg 
     5341 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag 
     5401 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct 
     5461 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta 
     5521 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg 
     5581 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta 
     5641 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc 
     5701 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt 
     5761 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata 
     5821 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg 
     5881 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc 
     5941 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg 
     6001 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct 
     6061 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 
     6121 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt 
     6181 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact 
     6241 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 
     6301 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 
     6361 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 
     6421 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 
     6481 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 
     6541 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 
     6601 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 
     6661 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 
     6721 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 
     6781 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 
     6841 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 
     6901 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 
     6961 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 
     7021 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 
     7081 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 
     7141 gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat aatattttat 
     7201 tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc 
     7261 cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct 
     7321 gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct 
     7381 gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa 
     7441 atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac 
     7501 atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt 
     7561 cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag 
     7621 ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc 
     7681 ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg 
     7741 aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt 
     7801 ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag 
     7861 cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat 
     7921 cagttttttc aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt 
     7981 ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc caattcactg 
     8041 ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag 
     8101 ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca 
     8161 acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac 
     8221 ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc 
     8281 ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg 
     8341 attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg 
     8401 gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact 
     8461 gaattaacgc cgaattaatt cgagctcgga tctgataatt tatttgaaaa ttcataagaa 
     8521 aagcaaacgt tacatgaatt gatgaaacaa tacaaagaca gataaagcca cgcacattta 
     8581 ggatattggc cgagattact gaatattgag taagatcacg gaatttctga caggagcatg 
     8641 tcttcaattc agcccaaatg gcagttgaaa tactcaaacc gccccatatg caggagcgga 
     8701 tcattcattg tttgtttggt tgcctttgcc aacatgggag tccaagattc tgcagttaga 
     8761 tctcggtgac gggcaggacc ggacggggcg gtaccggcag gctgaagtcc agctgccaga 
     8821 aacccacgtc atgccagttc ccgtgcttga agccggccgc ccgcagcatg ccgcgggggg 
     8881 catatccgag cgcctcgtgc atgcgcacgc tcgggtcgtt gggcagcccg atgacagcga 
     8941 ccacgctctt gaagccctgt gcctccaggg acttcagcag gtgggtgtag agcgtggagc 
     9001 ccagtcccgt ccgctggtgg cggggggaga cgtacacggt cgactcggcc gtccagtcgt 
     9061 aggcgttgcg tgccttccag gggcccgcgt aggcgatgcc ggcgacctcg ccgtccacct 
     9121 cggcgacgag ccagggatag cgctcccgca gacggacgag gtcgtccgtc cactcctgcg 
     9181 gttcctgcgg ctcggtacgg aagttgaccg tgcttgtctc gatgtagtgg ttgacgatgg 
     9241 tgcagaccgc cggcatgtcc gcctcggtgg cacggcggat gtcggccggg cgtcgttctg 
     9301 ggctcatcga ttcgatttgg tgtatcgaga ttggttatga aattcagatg ctagtgtaat 
     9361 gtattggtaa tttgggaaga tataatagga agcaaggcta tttatccatt tctgaaaagg 
     9421 cgaaatggcg tcaccgcgag cgtcacgcgc attccgttct tgctgtaaag cgttgtttgg 
     9481 tacacttttg actagcgagg cttggcgtgt cagcgtatct attcaaaagt cgttaatggc 
     9541 tgcggatcaa gaaaaagttg gaatagaaac agaatacccg cgaaattcag gcccggttgc 
     9601 catgtcctac acgccgaaat aaacgaccaa attagtagaa aaataaaaac tagctcagat 
     9661 acttacgtca cgtcttgcgc actgatttga aaaatctcag aattcgagct 
//
