LOCUS ER-gb 9710 bp DNA circular 11-OCT-2007 DEFINITION pAN142. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|446465895| COMMENT VNTDBDATE|459947555| COMMENT VNTNAME|ER-gb| COMMENT VNTAUTHORNAME|NebenfŸhr Lab| COMMENT VNTAUTHORTEL|(865) 974-9201| COMMENT VNTAUTHORWWW|http://botany1.bio.utk.edu| COMMENT VNTAUTHORAD1|Univeristy of Tennessee| COMMENT VNTAUTHORAD2|Department of Botany| COMMENT VNTAUTHORAD3|1406 Circle Dr| COMMENT VNTAUTHORAD4|Knoxville, TN 37996-1100| FEATURES Location/Qualifiers repeat_region 8384..8410 /vntifkey="34" /label=LB /note="left border repeat from C58 T-DNA" polyA_signal complement(8494..8745) /vntifkey="25" /label=PolyA_Signal_1 /note="from mannopine synthase" gene complement(8756..9307) /gene="BAR" /vntifkey="60" CDS complement(8756..9307) /gene="BAR" /codon_start=1 /product="phosphinothricin acetyl transferase" /protein_id="AAP78910.1" /db_xref="GI:32265028" /vntifkey="4" /label=BAR-R /note="basta resistance protein" promoter complement(9313..9693) /vntifkey="30" /label=Promoter\(bar) /note="mannopine synthase promoter" repeat_region 2106..2129 /vntifkey="34" /label=RB /note="right border repeat from C58 T-DNA" rep_origin complement(3191..4191) /vntifkey="33" /label=Rep_Origin_1 /note="pVS1" rep_origin complement(6594..6875) /vntifkey="33" /label=Rep_Origin_2 /note="pBR325" gene complement(7165..7959) /gene="aadA" /vntifkey="60" CDS complement(7165..7959) /gene="aadA" /function="kanamycin resistance" /codon_start=1 /product="aminoglycoside phosphotransferase" /protein_id="AAP78911.1" /db_xref="GI:32265029" /vntifkey="4" /label=KAN-R /note="aadA" promoter 10..736 /vntifkey="29" /label=d35S /note="double 35S promoter" terminator 1616..1865 /vntifkey="43" /label=nos\3' /note="terminator from nopaline synthase gene" primer_bind 672..695 /vntifkey="28" /label=35S\primer sig_peptide 788..856 /vntifkey="94" /label=signal\peptide CDS 788..1609 /vntifkey="4" /label=ER-GFP5 /note="WAK2 signal peptide + GFP5 + HDEL" BASE COUNT 2414 a 2609 c 2587 g 2100 t ORIGIN 1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 721 tcatttcatt tggagaggac agcccagatc aactagtctt aagtccggag ctagctctag 781 agagaagatg aaggtacagg agggtttgtt cgtggtggct gttttctacc ttgcttatac 841 gcagctagtc aaggggcaac ctcgcaagga gtgcggatcc atgggtaaag gagaactttt 901 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc 961 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg 1021 cactactgga aaactacctg ttccctggcc aacacttgtc actactttct cttatggtgt 1081 tcaatgcttt tcaagatacc cagatcatat gaagcggcac gacttcttca agagcgccat 1141 gcctgaggga tacgtgcagg agaggaccat cttcttcaag gacgacggga actacaagac 1201 acgtgctgaa gtcaagtttg agggagacac cctcgtcaac aggatcgagc ttaagggaat 1261 cgatttcaag gaggacggaa acatcctcgg ccacaagttg gaatacaact acaactccca 1321 caacgtatac atcatggccg acaagcaaaa gaacggcatc aaagccaact tcaagacccg 1381 ccacaacatc gaagacggcg gcgtgcaact cgctgatcat tatcaacaaa atactccaat 1441 tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc 1501 gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg 1561 gattacacat ggcatggatg aactatacag atctcatgac gagctgtaac tgcagttcaa 1621 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca 1681 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat 1741 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa 1801 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag 1861 atcgggaatt cgatatcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa 1921 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 1981 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 2041 tgctagagca gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca 2101 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataac 2161 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac 2221 cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag 2281 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag 2341 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc 2401 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg 2461 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac 2521 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca 2581 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga 2641 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc 2701 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca 2761 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc 2821 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg 2881 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg 2941 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc 3001 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc 3061 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg 3121 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat 3181 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt 3241 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag 3301 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa 3361 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa 3421 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc 3481 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat 3541 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc 3601 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac 3661 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca 3721 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac 3781 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt 3841 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc 3901 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact 3961 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc 4021 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa 4081 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg 4141 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg 4201 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc 4261 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga 4321 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc 4381 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc 4441 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg acggtcgcaa 4501 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa 4561 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg 4621 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc 4681 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta 4741 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa 4801 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt 4861 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt 4921 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg 4981 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa 5041 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca 5101 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat 5161 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct 5221 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca 5281 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg 5341 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag 5401 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct 5461 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta 5521 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg 5581 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta 5641 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc 5701 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt 5761 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata 5821 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg 5881 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc 5941 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg 6001 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct 6061 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 6121 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt 6181 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact 6241 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 6301 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 6361 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 6421 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 6481 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 6541 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 6601 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 6661 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 6721 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 6781 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 6841 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 6901 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 6961 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 7021 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 7081 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 7141 gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat aatattttat 7201 tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc 7261 cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct 7321 gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct 7381 gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa 7441 atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac 7501 atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt 7561 cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag 7621 ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc 7681 ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg 7741 aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt 7801 ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag 7861 cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat 7921 cagttttttc aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt 7981 ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc caattcactg 8041 ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag 8101 ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca 8161 acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac 8221 ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc 8281 ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg 8341 attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg 8401 gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact 8461 gaattaacgc cgaattaatt cgagctcgga tctgataatt tatttgaaaa ttcataagaa 8521 aagcaaacgt tacatgaatt gatgaaacaa tacaaagaca gataaagcca cgcacattta 8581 ggatattggc cgagattact gaatattgag taagatcacg gaatttctga caggagcatg 8641 tcttcaattc agcccaaatg gcagttgaaa tactcaaacc gccccatatg caggagcgga 8701 tcattcattg tttgtttggt tgcctttgcc aacatgggag tccaagattc tgcagttaga 8761 tctcggtgac gggcaggacc ggacggggcg gtaccggcag gctgaagtcc agctgccaga 8821 aacccacgtc atgccagttc ccgtgcttga agccggccgc ccgcagcatg ccgcgggggg 8881 catatccgag cgcctcgtgc atgcgcacgc tcgggtcgtt gggcagcccg atgacagcga 8941 ccacgctctt gaagccctgt gcctccaggg acttcagcag gtgggtgtag agcgtggagc 9001 ccagtcccgt ccgctggtgg cggggggaga cgtacacggt cgactcggcc gtccagtcgt 9061 aggcgttgcg tgccttccag gggcccgcgt aggcgatgcc ggcgacctcg ccgtccacct 9121 cggcgacgag ccagggatag cgctcccgca gacggacgag gtcgtccgtc cactcctgcg 9181 gttcctgcgg ctcggtacgg aagttgaccg tgcttgtctc gatgtagtgg ttgacgatgg 9241 tgcagaccgc cggcatgtcc gcctcggtgg cacggcggat gtcggccggg cgtcgttctg 9301 ggctcatcga ttcgatttgg tgtatcgaga ttggttatga aattcagatg ctagtgtaat 9361 gtattggtaa tttgggaaga tataatagga agcaaggcta tttatccatt tctgaaaagg 9421 cgaaatggcg tcaccgcgag cgtcacgcgc attccgttct tgctgtaaag cgttgtttgg 9481 tacacttttg actagcgagg cttggcgtgt cagcgtatct attcaaaagt cgttaatggc 9541 tgcggatcaa gaaaaagttg gaatagaaac agaatacccg cgaaattcag gcccggttgc 9601 catgtcctac acgccgaaat aaacgaccaa attagtagaa aaataaaaac tagctcagat 9661 acttacgtca cgtcttgcgc actgatttga aaaatctcag aattcgagct //